BMRB Entry 16706

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16706

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
1H, 13C, and 15N chemical shift assignments for H2H2, a mini prion protein
Deposition date:
2010-02-04
Original release date:
2010-03-31
Authors:
Pastore, Annalisa; Pauwels, Kris; Adrover, Miquel; de Chiara, Cesira; Prigent, Stephanie; Rezeai, Human
Citation:

Citation: Adrover, Miquel; Pauwels, Kris; Prigent, Stephanie; de Chiara, Cesira; Xu, Zhou; Chapuis, Celine; Pastore, Annalisa; Rezaei, Human. "Prion fibrillization is mediated by a native structural element that comprises helices H2 and H3."  J. Biol. Chem. 285, 21004-21012 (2010).
PubMed: 20375014

Assembly members:

Assembly members:
H2H3, polymer, 89 residues, 10227.3 Da.

Natural source:

Natural source:   Common Name: sheep   Taxonomy ID: 9940   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Ovis aries

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
H2H3: MGSSHHHHHHSSGLVPRGSH MRPVDQYSNQNNFVHDCVNI TVKQHTVTTTTKGENFTETD IKAMERVVEQMCITQYQRES QAYYQRGAS

Data sets:
Data typeCount
13C chemical shifts194
15N chemical shifts72
1H chemical shifts420

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1H2H31

Entities:

Entity 1, H2H3 89 residues - 10227.3 Da.

The first 21 residues represents a non-native his-tag. Residues 22-89 represent residues 167-234 of the native ovine PrP.

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYLEUVALPROARGGLYSERHIS
3   METARGPROVALASPGLNTYRSERASNGLN
4   ASNASNPHEVALHISASPCYSVALASNILE
5   THRVALLYSGLNHISTHRVALTHRTHRTHR
6   THRLYSGLYGLUASNPHETHRGLUTHRASP
7   ILELYSALAMETGLUARGVALVALGLUGLN
8   METCYSILETHRGLNTYRGLNARGGLUSER
9   GLNALATYRTYRGLNARGGLYALASER

Related Database Links:

PDB 2KTM
GB AAQ81754 AIE40054

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks