BMRB Entry 16809
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PDB ID: 2k7a
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16809
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Severin, Andrew; Fulton, Donald; Andreotti, Amy. "Solution Structure of the binary complex between the SH3 and SH2 domain of interleukin-2 tyrosine kinase" .
Assembly members:
Itk_SH3_domain, polymer, 63 residues, 7356.008 Da.
Itk_SH2_domain, polymer, 108 residues, 12534.314 Da.
Natural source: Common Name: house mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pgex2T
Entity Sequences (FASTA):
Itk_SH3_domain: GSPEETLVIALYDYQTNDPQ
ELALRCDEEYYLLDSSEIHW
WRVQDKNGHEGYAPSSYLVE
KSP
Itk_SH2_domain: NNLETYEWYNKSISRDKAEK
LLLDTGKEGAFMVRDSRTPG
TYTVSVFTKAIISENPCIKH
YHIKETNDSPKRYYVAEKYV
FDSIPLLIQYHQYNGGGLVT
RLRYPVCG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 1330 |
| 15N chemical shifts | 358 |
| 1H chemical shifts | 2088 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Itk_SH3_domain | 1 |
| 2 | Itk_SH2_domain | 2 |
Entities:
Entity 1, Itk_SH3_domain 63 residues - 7356.008 Da.
GS is the remnant from GST tag cleavage.
| 1 | GLY | SER | PRO | GLU | GLU | THR | LEU | VAL | ILE | ALA | ||||
| 2 | LEU | TYR | ASP | TYR | GLN | THR | ASN | ASP | PRO | GLN | ||||
| 3 | GLU | LEU | ALA | LEU | ARG | CYS | ASP | GLU | GLU | TYR | ||||
| 4 | TYR | LEU | LEU | ASP | SER | SER | GLU | ILE | HIS | TRP | ||||
| 5 | TRP | ARG | VAL | GLN | ASP | LYS | ASN | GLY | HIS | GLU | ||||
| 6 | GLY | TYR | ALA | PRO | SER | SER | TYR | LEU | VAL | GLU | ||||
| 7 | LYS | SER | PRO |
Entity 2, Itk_SH2_domain 108 residues - 12534.314 Da.
| 1 | ASN | ASN | LEU | GLU | THR | TYR | GLU | TRP | TYR | ASN | ||||
| 2 | LYS | SER | ILE | SER | ARG | ASP | LYS | ALA | GLU | LYS | ||||
| 3 | LEU | LEU | LEU | ASP | THR | GLY | LYS | GLU | GLY | ALA | ||||
| 4 | PHE | MET | VAL | ARG | ASP | SER | ARG | THR | PRO | GLY | ||||
| 5 | THR | TYR | THR | VAL | SER | VAL | PHE | THR | LYS | ALA | ||||
| 6 | ILE | ILE | SER | GLU | ASN | PRO | CYS | ILE | LYS | HIS | ||||
| 7 | TYR | HIS | ILE | LYS | GLU | THR | ASN | ASP | SER | PRO | ||||
| 8 | LYS | ARG | TYR | TYR | VAL | ALA | GLU | LYS | TYR | VAL | ||||
| 9 | PHE | ASP | SER | ILE | PRO | LEU | LEU | ILE | GLN | TYR | ||||
| 10 | HIS | GLN | TYR | ASN | GLY | GLY | GLY | LEU | VAL | THR | ||||
| 11 | ARG | LEU | ARG | TYR | PRO | VAL | CYS | GLY |
Related Database Links:
| BMRB | 11018 15912 15912 5461 |
| PDB | 1AWJ 2K79 2K7A 2RN8 2RNA 1LUI 1LUK 1LUM 1LUN 2ETZ 2EU0 3S9K |
| DBJ | BAA03129 BAE32118 BAA03129 BAC29830 BAE32118 |
| GB | AAA39337 AAA40518 AAI28375 AAI28376 EDL33821 AAA39337 AAA40518 AAI28375 AAI28376 EDL33821 |
| REF | NP_001102295 NP_001268894 NP_001268895 NP_001268896 NP_001268897 NP_001102295 NP_001268894 NP_001268895 NP_001268897 NP_034713 |
| SP | Q03526 Q03526 |
| AlphaFold | Q03526 Q03526 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks