BMRB Entry 16921

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16921

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Title:
1H, 13C, 15N chemical shifts for the bacterial cell division regulator MinE.
Deposition date:
2010-05-11
Original release date:
2010-07-26
Authors:
Ducat, Thierry; Goto, Natalie
Citation:

Citation: Ducat, Thierry; Goto, Natalie. "1H, 13C, 15N chemical shift assignments for the Neisseria gonorrhoeae MinE regulator of cell division septum placement."  Biomol. NMR Assignments 4, 227-229 (2010).
PubMed: 20623208

Assembly members:

Assembly members:
MinE, polymer, 95 residues, 11048 Da.

Natural source:

Natural source:   Common Name: Neisseria gonorrhoeae   Taxonomy ID: 485   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Neisseria gonorrhoeae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pEE46A

Entity Sequences (FASTA):

Entity Sequences (FASTA):
MinE: MSLIELLFGRKQKTATVARD RLQIIIAQERAQEGQTPDYL PTLRKALMEVLSKYVNVSLD NIRISQEKQDGMDVLELNIT LPEQKKVLEHHHHHH

Data sets:
Data typeCount
13C chemical shifts377
15N chemical shifts95
1H chemical shifts640

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1MinE, chain 11
2MinE, chain 21

Entities:

Entity 1, MinE, chain 1 95 residues - 11048 Da.

Two non-native residues (introduced from sub-cloning) plus 6 histidine residues (for purification) included at the C-terminus of the MinE sequence from N. gonorrhoeae.

1   METSERLEUILEGLULEULEUPHEGLYARG
2   LYSGLNLYSTHRALATHRVALALAARGASP
3   ARGLEUGLNILEILEILEALAGLNGLUARG
4   ALAGLNGLUGLYGLNTHRPROASPTYRLEU
5   PROTHRLEUARGLYSALALEUMETGLUVAL
6   LEUSERLYSTYRVALASNVALSERLEUASP
7   ASNILEARGILESERGLNGLULYSGLNASP
8   GLYMETASPVALLEUGLULEUASNILETHR
9   LEUPROGLUGLNLYSLYSVALLEUGLUHIS
10   HISHISHISHISHIS

Related Database Links:

PDB 2KXO
EMBL CAM07417 CEZ97582 CFA84494 CFB79676 CFC32949
GB AAK30127 AAW90432 ACF31058 AKP11296 AKP13283
REF WP_002246740 WP_003690052 WP_003749826 YP_208844
SP P58152 Q5F5V5 Q9JX18
AlphaFold P58152 Q5F5V5 Q9JX18

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks