BMRB Entry 16936
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PDB ID: 2ky8
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR16936
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Scarsdale, J. Neel; Webb, Heather; Ginder, Gordon; Williams, David. "Solution structure and dynamic analysis of chicken MBD2 methyl binding domain bound to a target-methylated DNA sequence." Nucleic Acids Res. 39, 6741-6752 (2011).
PubMed: 21531701
Assembly members:
cMBD2, polymer, 72 residues, 7861.131 Da.
DNA-strand_1, polymer, 11 residues, 2794.885 Da.
DNA-strand_2, polymer, 11 residues, 3044.045 Da.
MN, non-polymer, 54.938 Da.
Natural source: Common Name: chicken Taxonomy ID: 9031 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Gallus gallus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET32a
Entity Sequences (FASTA):
cMBD2: GSDKQGRTDCPALPPGWKKE
EVIRKSGLSAGKSDVYYFSP
SGKKFRSKPQLARYLGNAVD
LSCFDFRTGKMM
DNA-strand_1: GGAATXGGCXC
DNA-strand_2: GAGCXGATXCC
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 307 |
| 15N chemical shifts | 70 |
| 1H chemical shifts | 582 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | cMBD2 | 1 |
| 2 | DNA-strand_1 | 2 |
| 3 | DNA-strand_2 | 3 |
| 4 | MANGANESE (II) ION_1 | 4 |
| 5 | MANGANESE (II) ION_2 | 4 |
Entities:
Entity 1, cMBD2 72 residues - 7861.131 Da.
| 1 | GLY | SER | ASP | LYS | GLN | GLY | ARG | THR | ASP | CYS | ||||
| 2 | PRO | ALA | LEU | PRO | PRO | GLY | TRP | LYS | LYS | GLU | ||||
| 3 | GLU | VAL | ILE | ARG | LYS | SER | GLY | LEU | SER | ALA | ||||
| 4 | GLY | LYS | SER | ASP | VAL | TYR | TYR | PHE | SER | PRO | ||||
| 5 | SER | GLY | LYS | LYS | PHE | ARG | SER | LYS | PRO | GLN | ||||
| 6 | LEU | ALA | ARG | TYR | LEU | GLY | ASN | ALA | VAL | ASP | ||||
| 7 | LEU | SER | CYS | PHE | ASP | PHE | ARG | THR | GLY | LYS | ||||
| 8 | MET | MET |
Entity 2, DNA-strand_1 11 residues - 2794.885 Da.
| 1 | DG | DG | DA | DA | DT | 5CM | DG | DG | DC | THY | ||||
| 2 | DC |
Entity 3, DNA-strand_2 11 residues - 3044.045 Da.
| 1 | DG | DA | DG | DC | 5CM | DG | DA | DT | THY | DC | ||||
| 2 | DC |
Entity 4, MANGANESE (II) ION_1 - Mn - 54.938 Da.
| 1 | MN |
Related Database Links:
| GB | NP_001012403 AAC68872 AAC68874 AAD50372 AAD50373 AAH46607 |
| PDB | 2KY8 |
| REF | NP_001012403 NP_001108497 NP_001298000 NP_034903 XP_005074763 |
| SP | Q9Z2E1 |
| AlphaFold | Q9Z2E1 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
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SPARKY: Backbone
or all simulated peaks