BMRB Entry 17082

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PDB ID: 2l1e
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17082
MolProbity Validation Chart

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Title:
Mouse Prion Protein (121-231) with mutation F175A
Deposition date:
2010-07-28
Original release date:
2012-08-02
Authors:
Christen, Barbara; Damberger, Fred; Hornemann, Simone; Wuthrich, Kurt
Citation:

Citation: Christen, Barbara; Hornemann, Simone; Damberger, Fred; Wuthrich, Kurt. "Prion protein mPrP[F175A](121-231): structure and stability in solution."  J. Mol. Biol. 423, 496-502 (2012).
PubMed: 22922482

Assembly members:

Assembly members:
mPrP121-231_F175A, polymer, 114 residues, 13330.79 Da.

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pRSETA

Entity Sequences (FASTA):

Entity Sequences (FASTA):
mPrP121-231_F175A: GSVVGGLGGYMLGSAMSRPM IHFGNDWEDRYYRENMYRYP NQVYYRPVDQYSNQNNAVHD CVNITIKQHTVTTTTKGENF TETDVKMMERVVEQMCVTQY QKESQAYYDGRRSS

Data sets:
Data typeCount
13C chemical shifts479
15N chemical shifts135
1H chemical shifts797

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1prion1

Entities:

Entity 1, prion 114 residues - 13330.79 Da.

1   GLYSERVALVALGLYGLYLEUGLYGLYTYR
2   METLEUGLYSERALAMETSERARGPROMET
3   ILEHISPHEGLYASNASPTRPGLUASPARG
4   TYRTYRARGGLUASNMETTYRARGTYRPRO
5   ASNGLNVALTYRTYRARGPROVALASPGLN
6   TYRSERASNGLNASNASNALAVALHISASP
7   CYSVALASNILETHRILELYSGLNHISTHR
8   VALTHRTHRTHRTHRLYSGLYGLUASNPHE
9   THRGLUTHRASPVALLYSMETMETGLUARG
10   VALVALGLUGLNMETCYSVALTHRGLNTYR
11   GLNLYSGLUSERGLNALATYRTYRASPGLY
12   ARGARGSERSER

Related Database Links:

BMRB 15845 16071 16075 16076 16077 16078 16079 16080 16184 16185 16722 16723 17081 17084 17174 17213 17758 17759
PDB 1AG2 1XYX 1Y15 1Y16 2K5O 2KFM 2KFO 2KU5 2KU6 2L1D 2L1E 2L1H 2L39 2L40 4H88 4MA7 4MA8
DBJ BAA08790 BAE28320 BAE28693 BAE29994 BAE34221
EMBL CAJ18553
GB AAA39996 AAA39997 AAA39998 AAA41947 AAB30728
REF NP_001265185 NP_035300 NP_036763 XP_006235124
SP P04925 P13852 Q9Z0T3
AlphaFold P04925 P13852 Q9Z0T3

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