BMRB Entry 17112

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17112

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Title:
1H, 13C and 15N NMR Assignments of the C1B Subdomains of PKC-delta.
Deposition date:
2010-08-10
Original release date:
2010-12-08
Authors:
Ziemba, Brian; Jones, David; Booth, Jamie
Citation:

Citation: Ziemba, Brian; Booth, Jamie; Jones, David. "1H, 13C and 15N NMR assignments of the C1A and C1B subdomains of PKC-delta."  Biomol. NMR Assignments 5, 125-129 (2011).
PubMed: 21132404

Assembly members:

Assembly members:
PKC_delta_C1B, polymer, 82 residues, 9408.8 Da.
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Norway rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-4T1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PKC_delta_C1B: GSPEFIFQKERFNIDMPHRF KVYNYMSPTFCDHCGSLLWG LVKQGLKCEDCGMNVHHKCR EKVANLCGINQKVDSSGRIV TD

Data sets:
Data typeCount
13C chemical shifts319
15N chemical shifts73
1H chemical shifts441

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1C1B construct1
2Zinc 12
3Zinc 22

Entities:

Entity 1, C1B construct 82 residues - 9408.8 Da.

Residues 214-218 and 286-295 are derived from pGEX-4T1 vector. Residues 219-285 are from PKC-delta, and 231-280 is the 50 residue C1B domain.

1   GLYSERPROGLUPHEILEPHEGLNLYSGLU
2   ARGPHEASNILEASPMETPROHISARGPHE
3   LYSVALTYRASNTYRMETSERPROTHRPHE
4   CYSASPHISCYSGLYSERLEULEUTRPGLY
5   LEUVALLYSGLNGLYLEULYSCYSGLUASP
6   CYSGLYMETASNVALHISHISLYSCYSARG
7   GLULYSVALALAASNLEUCYSGLYILEASN
8   GLNLYSVALASPSERSERGLYARGILEVAL
9   THRASP

Entity 2, Zinc 1 - Zn - 65.409 Da.

1   ZN

Related Database Links:

PDB 1PTQ 1PTR 3UEJ 3UGI 3UGL

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks