BMRB Entry 17113

Name: 1H, 13C and 15N NMR Assignments of the C1A Subdomains of PKC-delta.
Published: 2010-12-08

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17113

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C and 15N NMR Assignments of the C1A Subdomains of PKC-delta.

Deposition date:

2010-08-10

Original release date:

2010-12-08

Authors:

Ziemba, Brian; Jones, David

Citation:

Citation: Brian, P. Ziemba; Jamie, C. Booth; David, Jones. "1H, 13C and 15N NMR assignments of the C1A and C1B subdomains of PKC-delta." Biomol. NMR Assignments 5, 125-129 (2011).
PubMed: 21132404

Assembly members:

Assembly members:
PKC_delta_C1A, polymer, 80 residues, 8977.2 Da.
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: Norway Rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norvegicus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-4T1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PKC_delta_C1A: GSPEFHYIKNHEFIATFFGQ PTFCSVCKEFVWGLNKQGYK CRQCNAAIHKKCIDKIIGRC TGTATNSRDTVDSSGRIVTD

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	295
15N chemical shifts	72
1H chemical shifts	439

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	C1A construct	1
2	Zinc_1	2
3	Zinc_2	2

Entities:

Entity 1, C1A construct 80 residues - 8977.2 Da.

The C1A construct contains a total of 80 amino acids (labeled 149-228) derived from PKC-delta (residues 154-218) including the C1A domain (residues 159-208) and residues from the pGEX-4T1 vector (residues 149-153 and 219-228).

1	GLY	SER	PRO	GLU	PHE	HIS	TYR	ILE	LYS	ASN
2	HIS	GLU	PHE	ILE	ALA	THR	PHE	PHE	GLY	GLN
3	PRO	THR	PHE	CYS	SER	VAL	CYS	LYS	GLU	PHE
4	VAL	TRP	GLY	LEU	ASN	LYS	GLN	GLY	TYR	LYS
5	CYS	ARG	GLN	CYS	ASN	ALA	ALA	ILE	HIS	LYS
6	LYS	CYS	ILE	ASP	LYS	ILE	ILE	GLY	ARG	CYS
7	THR	GLY	THR	ALA	THR	ASN	SER	ARG	ASP	THR
8	VAL	ASP	SER	SER	GLY	ARG	ILE	VAL	THR	ASP

Entity 2, Zinc_1 - Zn - 65.409 Da.

1

ZN

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1
3D HNHA	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_2	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_1	isotropic	sample_conditions_1
2D (HB)CB(CGCDCE)HE	sample_2	isotropic	sample_conditions_1
2D (HB)CB(CGCD)HD	sample_2	isotropic	sample_conditions_1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks

BMRB Entry 17113

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

1	GLY	SER	PRO	GLU	PHE	HIS	TYR	ILE	LYS	ASN
2	HIS	GLU	PHE	ILE	ALA	THR	PHE	PHE	GLY	GLN
3	PRO	THR	PHE	CYS	SER	VAL	CYS	LYS	GLU	PHE
4	VAL	TRP	GLY	LEU	ASN	LYS	GLN	GLY	TYR	LYS
5	CYS	ARG	GLN	CYS	ASN	ALA	ALA	ILE	HIS	LYS
6	LYS	CYS	ILE	ASP	LYS	ILE	ILE	GLY	ARG	CYS
7	THR	GLY	THR	ALA	THR	ASN	SER	ARG	ASP	THR
8	VAL	ASP	SER	SER	GLY	ARG	ILE	VAL	THR	ASP

1	GLY	SER	PRO	GLU	PHE	HIS	TYR	ILE	LYS	ASN
2	HIS	GLU	PHE	ILE	ALA	THR	PHE	PHE	GLY	GLN
3	PRO	THR	PHE	CYS	SER	VAL	CYS	LYS	GLU	PHE
4	VAL	TRP	GLY	LEU	ASN	LYS	GLN	GLY	TYR	LYS
5	CYS	ARG	GLN	CYS	ASN	ALA	ALA	ILE	HIS	LYS
6	LYS	CYS	ILE	ASP	LYS	ILE	ILE	GLY	ARG	CYS
7	THR	GLY	THR	ALA	THR	ASN	SER	ARG	ASP	THR
8	VAL	ASP	SER	SER	GLY	ARG	ILE	VAL	THR	ASP

1	GLY	SER	PRO	GLU	PHE	HIS	TYR	ILE	LYS	ASN
2	HIS	GLU	PHE	ILE	ALA	THR	PHE	PHE	GLY	GLN
3	PRO	THR	PHE	CYS	SER	VAL	CYS	LYS	GLU	PHE
4	VAL	TRP	GLY	LEU	ASN	LYS	GLN	GLY	TYR	LYS
5	CYS	ARG	GLN	CYS	ASN	ALA	ALA	ILE	HIS	LYS
6	LYS	CYS	ILE	ASP	LYS	ILE	ILE	GLY	ARG	CYS
7	THR	GLY	THR	ALA	THR	ASN	SER	ARG	ASP	THR
8	VAL	ASP	SER	SER	GLY	ARG	ILE	VAL	THR	ASP