BMRB Entry 17134

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17134

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Title:
Solution structure of Opossum Domain 11
Deposition date:
2010-08-18
Original release date:
2014-03-04
Authors:
Williams, Christopher; Hoppe, Hans; Rezgui, Dellel; Rezgui, Madeline; Frago, Susana; Ellis, Rosamund; Wattana-Amorn, Pakorn; Prince, Stuart; Zaccheo, Oliver; Forbes, Briony; Jones, E.; Crump, Matthew; Bassim, A.
Citation:

Citation: Williams, Christopher; Hoppe, Hans; Rezgui, Dellel; Strickland, Madeline; Frago, Susana; Ellis, Rosamund; Wattana-Amorn, Pakorn; Prince, Stuart; Zaccheo, Oliver; Forbes, Briony; Jones, E.; Crump, Matthew; Hassan, A.; Grutzner, Frank; Nolan, Catherine; Mungall, Andrew. "An exon splice enhancer primes IGF2:IGF2R binding site structure and function evolution"  Science 338, 1209-1213 (2012).
PubMed: 23197533

Assembly members:

Assembly members:
IGF2R, polymer, 151 residues, 16103.299 Da.

Natural source:

Natural source:   Common Name: Gray Short-tailed Opossum   Taxonomy ID: 13616   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Monodelphis domestica

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET26a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
IGF2R: MKSNIQDNCQVTNPATGHLF DLNSLKNDSGYSVAYSEKGL IYIGICGGTKNCPSGVGVCF GLTKINAGSWNSQLMYVDQV LQLVYDDGAPCPSKNALKYK SVISFVCTHDSGANNKPVFV SLDKQTCTLYFSWHTPLACE KEEPRHHHHHH

Data sets:
Data typeCount
13C chemical shifts596
15N chemical shifts150
1H chemical shifts937

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1IGF2R1

Entities:

Entity 1, IGF2R 151 residues - 16103.299 Da.

Domain 11 of the IGF2R from Monodelphis domestica. The C-terminal residues EEVPRHHHHHH are a non-native affinity tag

1   METLYSSERASNILEGLNASPASNCYSGLN
2   VALTHRASNPROALATHRGLYHISLEUPHE
3   ASPLEUASNSERLEULYSASNASPSERGLY
4   TYRSERVALALATYRSERGLULYSGLYLEU
5   ILETYRILEGLYILECYSGLYGLYTHRLYS
6   ASNCYSPROSERGLYVALGLYVALCYSPHE
7   GLYLEUTHRLYSILEASNALAGLYSERTRP
8   ASNSERGLNLEUMETTYRVALASPGLNVAL
9   LEUGLNLEUVALTYRASPASPGLYALAPRO
10   CYSPROSERLYSASNALALEULYSTYRLYS
11   SERVALILESERPHEVALCYSTHRHISASP
12   SERGLYALAASNASNLYSPROVALPHEVAL
13   SERLEUASPLYSGLNTHRCYSTHRLEUTYR
14   PHESERTRPHISTHRPROLEUALACYSGLU
15   LYSGLUGLUPROARGHISHISHISHISHIS
16   HIS

Related Database Links:

PDB 2L2G

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