BMRB Entry 17160

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PDB ID: 2lhs
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17160
MolProbity Validation Chart

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Title:
1H, 13C, 15N resonance assignment of the chitin-binding protein CBP21 from Serratia marcescens
Deposition date:
2010-08-31
Original release date:
2010-11-09
Authors:
Vaaje-Kolstad, Gustav; Eijsink, Vincent; Aachmann, Finn
Citation:

Citation: Aachmann, Finn; Eijsink, Vincent; Vaaje-Kolstad, Gustav. "(1)H, (13)C, (15)N resonance assignment of the chitin-binding protein CBP21 from Serratia marcescens."  Biomol. NMR Assignments 5, 117-119 (2011).
PubMed: 21052875

Assembly members:

Assembly members:
CBP21, polymer, 170 residues, 18794 Da.

Natural source:

Natural source:   Common Name: Serratia marcescens   Taxonomy ID: 615   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Serratia marcescens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pRSETB_cbp21

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CBP21: HGYVESPASRAYQCKLQLNT QCGSVQYEPQSVEGLKGFPQ AGPADGHIASADKSTFFELD QQTPTRWNKLNLKTGPNSFT WKLTARHSTTSWRYFITKPN WDASQPLTRASFDLTPFCQF NDGGAIPAAQVTHQCNIPAD RSGSHVILAVWDIADTANAF YQAIDVNLSK

Data sets:
Data typeCount
13C chemical shifts652
15N chemical shifts179
1H chemical shifts1032

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1CBP211

Entities:

Entity 1, CBP21 170 residues - 18794 Da.

CBP21 carrying an N-terminal leader sequence, which is cleaved of during translocation to the periplasmic space resulting in CBP21 (AA: 28-197)

1   HISGLYTYRVALGLUSERPROALASERARG
2   ALATYRGLNCYSLYSLEUGLNLEUASNTHR
3   GLNCYSGLYSERVALGLNTYRGLUPROGLN
4   SERVALGLUGLYLEULYSGLYPHEPROGLN
5   ALAGLYPROALAASPGLYHISILEALASER
6   ALAASPLYSSERTHRPHEPHEGLULEUASP
7   GLNGLNTHRPROTHRARGTRPASNLYSLEU
8   ASNLEULYSTHRGLYPROASNSERPHETHR
9   TRPLYSLEUTHRALAARGHISSERTHRTHR
10   SERTRPARGTYRPHEILETHRLYSPROASN
11   TRPASPALASERGLNPROLEUTHRARGALA
12   SERPHEASPLEUTHRPROPHECYSGLNPHE
13   ASNASPGLYGLYALAILEPROALAALAGLN
14   VALTHRHISGLNCYSASNILEPROALAASP
15   ARGSERGLYSERHISVALILELEUALAVAL
16   TRPASPILEALAASPTHRALAASNALAPHE
17   TYRGLNALAILEASPVALASNLEUSERLYS

Related Database Links:

PDB 2BEM 2BEN 2LHS
DBJ BAA31569 BAO35063
EMBL CDG13442
GB AAU88202 AGO05942 AIA47583 AIM23065 AKL42792
REF WP_004936574 WP_019453872 WP_025303806 WP_033635482 WP_033643984

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