BMRB Entry 17216

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17216

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Title:
How the HIV-1 Nucleocapsid Protein Binds and Destabilises the (-)Primer Binding Site During Reverse Transcription
Deposition date:
2010-09-29
Original release date:
2010-11-10
Authors:
Bourbigot, Sarah; Ramalanjaona, Nick; Boudier, Christian; Salgado, Gilmar; Roques, Bernard; Mely, Yves; Bouaziz, Serge; Morellet, Nelly
Citation:

Citation: Bourbigot, Sarah; Ramalanjaona, Nick; Boudier, Christian; Salgado, Gilmar; Roques, Bernard; Mely, Yves; Bouaziz, Serge; Morellet, Nelly. "How the HIV-1 Nucleocapsid Protein Binds and Destabilises the (-)Primer Binding Site During Reverse Transcription"  J. Mol. Biol. 383, 1112-1128 (2008).
PubMed: 18773912

Assembly members:

Assembly members:
PPBS, polymer, 12 residues, Formula weight is not available
NCp7(12-55), polymer, 44 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human immunodeficiency virus 1   Taxonomy ID: 11676   Superkingdom: not available   Kingdom: not available   Genus/species: Lentivirus not available

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PPBS: GCCCTGTTGGGC
NCp7(12-55): NVKCFNCGKEGHTARNCRAP RKKGCWKCGKEGHQMKDCTE RQAN

Data sets:
Data typeCount
kinetic rates4

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1PPBS1
2NCp7(12-55)2

Entities:

Entity 1, PPBS 12 residues - Formula weight is not available

1   DGDCDCDCDTDGDTDTDGDG
2   DGDC

Entity 2, NCp7(12-55) 44 residues - Formula weight is not available

1   ASNVALLYSCYSPHEASNCYSGLYLYSGLU
2   GLYHISTHRALAARGASNCYSARGALAPRO
3   ARGLYSLYSGLYCYSTRPLYSCYSGLYLYS
4   GLUGLYHISGLNMETLYSASPCYSTHRGLU
5   ARGGLNALAASN

Related Database Links:

BMRB 2217 25532
PDB 1BJ6 1ESK 1Q3Y 1Q3Z 2EXF 2JZW 2L4L
DBJ BAA12988 BAA12996 BAB85751 BAB85752 BAC02530
EMBL CAB58976 CAB58988 CAB98186 CAP69989 CAP69993
GB AAA44201 AAA45076 AAA76686 AAB05598 AAB21888
PIR FOVWLV
PRF 1102247B 1103299C
REF NP_057849 NP_057850 NP_579881
SP P03347 P03348 P03366 P03367 P04585
AlphaFold P03347 P03348 P03366 P03367 P04585