BMRB Entry 17249

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PDB ID: 2ml2
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17249
MolProbity Validation Chart

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Title:
1H, 13C and 15N resonances of the AlgE62 subunit from Azotobacter vinelandii mannuronan C5-epimerase
Deposition date:
2010-10-12
Original release date:
2011-01-18
Authors:
Andressen, Trygve; Buchinger, Edith; Skjaak-Break, Gudmund; Valla, Svein; Aachmann, Finn
Citation:

Citation: Andreassen, Trygve; Buchinger, Edith; Skjak-Braek, Gudmund; Valla, Svein; Aachmann, Finn. "1H, 13C and 15N resonances of the AlgE62 subunit from Azotobacter vinelandii mannuronan C5-epimerase."  Biomol. NMR Assignments 5, 147-149 (2011).
PubMed: 21188559

Assembly members:

Assembly members:
AlgE6R2, polymer, 161 residues, Formula weight is not available
CA, non-polymer, 40.078 Da.

Natural source:

Natural source:   Common Name: Azotobacter vinelandii   Taxonomy ID: 354   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Azotobacter vinelandii

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pFA12

Entity Sequences (FASTA):

Entity Sequences (FASTA):
AlgE6R2: DPSAEAQPIVGSDLDDQLHG TLLGEEISGGGGADQLYGYG GGDLLDGGAGRDRLTGGEGA DTFRFALREDSQRSAAGTFS DLILDFDPTQDKLDVSALGF TGLGNGYAGTLAVSVSDDGT RTYLKSYETDAEGRSFEVSL QGNHAAALSADNILFATPVP V

Data sets:
Data typeCount
13C chemical shifts606
15N chemical shifts160
1H chemical shifts941

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1R21
2calcium ion2

Entities:

Entity 1, R2 161 residues - Formula weight is not available

1   ASPPROSERALAGLUALAGLNPROILEVAL
2   GLYSERASPLEUASPASPGLNLEUHISGLY
3   THRLEULEUGLYGLUGLUILESERGLYGLY
4   GLYGLYALAASPGLNLEUTYRGLYTYRGLY
5   GLYGLYASPLEULEUASPGLYGLYALAGLY
6   ARGASPARGLEUTHRGLYGLYGLUGLYALA
7   ASPTHRPHEARGPHEALALEUARGGLUASP
8   SERGLNARGSERALAALAGLYTHRPHESER
9   ASPLEUILELEUASPPHEASPPROTHRGLN
10   ASPLYSLEUASPVALSERALALEUGLYPHE
11   THRGLYLEUGLYASNGLYTYRALAGLYTHR
12   LEUALAVALSERVALSERASPASPGLYTHR
13   ARGTHRTYRLEULYSSERTYRGLUTHRASP
14   ALAGLUGLYARGSERPHEGLUVALSERLEU
15   GLNGLYASNHISALAALAALALEUSERALA
16   ASPASNILELEUPHEALATHRPROVALPRO
17   VAL

Entity 2, calcium ion - Ca - 40.078 Da.

1   CA

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