BMRB Entry 17258
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PDB ID: 2l4v
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17258
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
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Citation: Irene, Deli; Chen, Bo-Jiun; Lo, Si-Hung; Liu, Ting-Hang; Tzen, Jason T-C; Chyan, Chia-Lin. "Resonance assignments and secondary structure of a phytocystatin from Ananas comosus." Biomol. NMR Assignments 6, 99-101 (2012).
PubMed: 21814766
Assembly members:
Pineapple_Cystatin, polymer, 135 residues, 15472.320 Da.
Natural source: Common Name: pineapple Taxonomy ID: 4615 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Ananas comosus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a
Entity Sequences (FASTA):
Pineapple_Cystatin: MADEQPKKPVPAAEEAPAAE
AEAEEEEGLHLEDDQEPREH
PIMGGIYDAPLNNENGFDKE
DLARFAVREYNNKNNALLEF
VRVVKAKEQVVSGMMHYLTV
EVNDAGKKKLYEAKVWEQVW
MNFRQLQEFTYLGDA
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 549 |
| 15N chemical shifts | 144 |
| 1H chemical shifts | 959 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Pineapple Cystatin | 1 |
Entities:
Entity 1, Pineapple Cystatin 135 residues - 15472.320 Da.
| 1 | MET | ALA | ASP | GLU | GLN | PRO | LYS | LYS | PRO | VAL | ||||
| 2 | PRO | ALA | ALA | GLU | GLU | ALA | PRO | ALA | ALA | GLU | ||||
| 3 | ALA | GLU | ALA | GLU | GLU | GLU | GLU | GLY | LEU | HIS | ||||
| 4 | LEU | GLU | ASP | ASP | GLN | GLU | PRO | ARG | GLU | HIS | ||||
| 5 | PRO | ILE | MET | GLY | GLY | ILE | TYR | ASP | ALA | PRO | ||||
| 6 | LEU | ASN | ASN | GLU | ASN | GLY | PHE | ASP | LYS | GLU | ||||
| 7 | ASP | LEU | ALA | ARG | PHE | ALA | VAL | ARG | GLU | TYR | ||||
| 8 | ASN | ASN | LYS | ASN | ASN | ALA | LEU | LEU | GLU | PHE | ||||
| 9 | VAL | ARG | VAL | VAL | LYS | ALA | LYS | GLU | GLN | VAL | ||||
| 10 | VAL | SER | GLY | MET | MET | HIS | TYR | LEU | THR | VAL | ||||
| 11 | GLU | VAL | ASN | ASP | ALA | GLY | LYS | LYS | LYS | LEU | ||||
| 12 | TYR | GLU | ALA | LYS | VAL | TRP | GLU | GLN | VAL | TRP | ||||
| 13 | MET | ASN | PHE | ARG | GLN | LEU | GLN | GLU | PHE | THR | ||||
| 14 | TYR | LEU | GLY | ASP | ALA |
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