BMRB Entry 17315
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PDB ID: 2l6m
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17315
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Barraud, Pierre; Emmerth, Stephan; Shimada, Yukiko; Hotz, Hans-Rudolf; Allain, Frederic; Buhler, Marc. "An extended dsRBD with a novel zinc-binding motif mediates nuclear retention of fission yeast Dicer" EMBO J. 30, 4223-4235 (2011).
PubMed: 21847092
Assembly members:
dsRBD, polymer, 121 residues, 11806.978 Da.
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: fission yeast Taxonomy ID: 4896 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Schizosaccharomyces pombe
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a+
Entity Sequences (FASTA):
dsRBD: MGSSHHHHHHSSGLVPRGSH
MKGDIEHKVYQLLKDQGCED
FGTKCVIEEVKSSHKTLLNT
ELHLTKYYGFSFFRHGNIVA
YGKSRKVANAKYIMKQRLLK
LLEDKSNLLLYSCNCKFSKK
K
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 316 |
| 15N chemical shifts | 100 |
| 1H chemical shifts | 677 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | dsRBD | 1 |
| 2 | ZINC ION | 2 |
Entities:
Entity 1, dsRBD 121 residues - 11806.978 Da.
| 1 | MET | GLY | SER | SER | HIS | HIS | HIS | HIS | HIS | HIS | ||||
| 2 | SER | SER | GLY | LEU | VAL | PRO | ARG | GLY | SER | HIS | ||||
| 3 | MET | LYS | GLY | ASP | ILE | GLU | HIS | LYS | VAL | TYR | ||||
| 4 | GLN | LEU | LEU | LYS | ASP | GLN | GLY | CYS | GLU | ASP | ||||
| 5 | PHE | GLY | THR | LYS | CYS | VAL | ILE | GLU | GLU | VAL | ||||
| 6 | LYS | SER | SER | HIS | LYS | THR | LEU | LEU | ASN | THR | ||||
| 7 | GLU | LEU | HIS | LEU | THR | LYS | TYR | TYR | GLY | PHE | ||||
| 8 | SER | PHE | PHE | ARG | HIS | GLY | ASN | ILE | VAL | ALA | ||||
| 9 | TYR | GLY | LYS | SER | ARG | LYS | VAL | ALA | ASN | ALA | ||||
| 10 | LYS | TYR | ILE | MET | LYS | GLN | ARG | LEU | LEU | LYS | ||||
| 11 | LEU | LEU | GLU | ASP | LYS | SER | ASN | LEU | LEU | LEU | ||||
| 12 | TYR | SER | CYS | ASN | CYS | LYS | PHE | SER | LYS | LYS | ||||
| 13 | LYS |
Entity 2, ZINC ION - Zn - 65.409 Da.
| 1 | ZN |
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