BMRB Entry 17384

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17384

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Title:
Backbone Assignment of the Tyrosine Kinase Src Catalytic Domain in Complex with Imatinib.
Deposition date:
2010-12-28
Original release date:
2011-05-04
Authors:
Campos-Olivas, Ramon; Marenchino, Marco; Gervasio, Francesco
Citation:

Citation: Campos-Olivas, Ramon; Marenchino, Marco; Scapozza, Leonardo; Gervasio, Francesco. "Backbone assignment of the tyrosine kinase Src catalytic domain in complex with imatinib."  Biomol. NMR Assignments 5, 221-224 (2011).
PubMed: 21523440

Assembly members:

Assembly members:
SrcKD, polymer, 286 residues, Formula weight is not available
STI, non-polymer, 493.603 Da.

Natural source:

Natural source:   Common Name: chicken   Taxonomy ID: 9031   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Gallus gallus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: modifyed pET28

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SrcKD: GHMQTQGLAKDAWEIPRESL RLEVKLGQGCFGEVWMGTWN GTTRVAIKTLKPGTMSPEAF LQEAQVMKKLRHEKLVQLYA VVSEEPIYIVTEYMSKGSLL DFLKGEMGKYLRLPQLVDMA AQIASGMAYVERMNYVHRDL RAANILVGENLVCKVADFGL ARLIEDNEYTARQGAKFPIK WTAPEAALYGRFTIKSDVWS FGILLTELTTKGRVPYPGMV NREVLDQVERGYRMPCPPEC PESLHDLMCQCWRKDPEERP TFEYLQAFLEDYFTSTEPQY QPGENL

Data sets:
Data typeCount
13C chemical shifts685
15N chemical shifts215
1H chemical shifts215

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Src Kinase Domain1
2Imatinib/Gleevec2

Entities:

Entity 1, Src Kinase Domain 286 residues - Formula weight is not available

Residues -3 to -1 represent a non-native tag. Residues 251 to 533 correspond to the Src kinase catalytic domain

1   GLYHISMETGLNTHRGLNGLYLEUALALYS
2   ASPALATRPGLUILEPROARGGLUSERLEU
3   ARGLEUGLUVALLYSLEUGLYGLNGLYCYS
4   PHEGLYGLUVALTRPMETGLYTHRTRPASN
5   GLYTHRTHRARGVALALAILELYSTHRLEU
6   LYSPROGLYTHRMETSERPROGLUALAPHE
7   LEUGLNGLUALAGLNVALMETLYSLYSLEU
8   ARGHISGLULYSLEUVALGLNLEUTYRALA
9   VALVALSERGLUGLUPROILETYRILEVAL
10   THRGLUTYRMETSERLYSGLYSERLEULEU
11   ASPPHELEULYSGLYGLUMETGLYLYSTYR
12   LEUARGLEUPROGLNLEUVALASPMETALA
13   ALAGLNILEALASERGLYMETALATYRVAL
14   GLUARGMETASNTYRVALHISARGASPLEU
15   ARGALAALAASNILELEUVALGLYGLUASN
16   LEUVALCYSLYSVALALAASPPHEGLYLEU
17   ALAARGLEUILEGLUASPASNGLUTYRTHR
18   ALAARGGLNGLYALALYSPHEPROILELYS
19   TRPTHRALAPROGLUALAALALEUTYRGLY
20   ARGPHETHRILELYSSERASPVALTRPSER
21   PHEGLYILELEULEUTHRGLULEUTHRTHR
22   LYSGLYARGVALPROTYRPROGLYMETVAL
23   ASNARGGLUVALLEUASPGLNVALGLUARG
24   GLYTYRARGMETPROCYSPROPROGLUCYS
25   PROGLUSERLEUHISASPLEUMETCYSGLN
26   CYSTRPARGLYSASPPROGLUGLUARGPRO
27   THRPHEGLUTYRLEUGLNALAPHELEUGLU
28   ASPTYRPHETHRSERTHRGLUPROGLNTYR
29   GLNPROGLYGLUASNLEU

Entity 2, Imatinib/Gleevec - C29 H31 N7 O - 493.603 Da.

1   STI

Related Database Links:

PDB 1FMK 1KSW 1Y57 1YI6 1YOJ 1YOL 1YOM 2BDF 2BDJ 2H8H 2HWO 2HWP 2OIQ 2PTK 2QI8 2QLQ 2QQ7 2SRC 3D7T 3D7U 3DQW 3DQX 3EL7 3EL8 3EN4 3EN5 3EN6 3EN7 3F3T 3F3U 3F3V 3F3W 3F6X 3G5D 3G6G 3G6H 3GEQ 3LOK 3OEZ 3OF0 3QLF 3QLG 3SVV 3TZ7 3TZ8 3TZ9 3U4W 3U51 3UQF 3UQG 4AGW 4DGG 4FIC 4K11 4LGG 4LGH 4MCV 4MXO 4MXX 4MXY 4MXZ 4O2P 4U5J 5D10 5D11 5D12
DBJ BAB14871 BAE26865 BAI47379
EMBL CAA23696 CAA24495 CAA26485 CAA32012 CAA33404
GB AAA40135 AAA42563 AAA42565 AAA42583 AAA49961
PRF 0903255A
REF NP_001011413 NP_001020566 NP_001080738 NP_001104274 NP_001248263
SP P00523 P00524 P00525 P00526 P05480
TPG DAA23281
AlphaFold P00523 P00524 P00525 P00526 P05480

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks