BMRB Entry 17640

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR17640

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Title:
EL222(14-222)
Deposition date:
2011-05-12
Original release date:
2012-06-04
Authors:
Nash, Abigail; McNulty, Reginald; Shillito, Mary; Swartz, Trevor; Bogomolni, Roberto; Luecke, Hartmut; Gardner, Kevin
Citation:

Citation: Nash, Abigail; McNulty, Reginald; Shillito, Mary Elizabeth; Swartz, Trevor; Bogomolni, Roberto; Luecke, Hartmut; Gardner, Kevin. "Structural basis of photosensitivity in a bacterial light-oxygen-voltage/helix-turn-helix (LOV-HTH) DNA-binding protein."  Proc. Natl. Acad. Sci. U.S.A. 108, 9449-9454 (2011).
PubMed: 21606338

Assembly members:

Assembly members:
EL222(14-222), polymer, 212 residues, Formula weight is not available
FMN, non-polymer, 456.344 Da.

Natural source:

Natural source:   Common Name: Erythrobacter litoralis   Taxonomy ID: 314225   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Erythrobacter litoralis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: His6-GB1 parallel

Entity Sequences (FASTA):

Entity Sequences (FASTA):
EL222(14-222): GEFGADDTRVEVQPPAQWVL DLIEASPIASVVSDPRLADN PLIAINQAFTDLTGYSEEEC VGRNCRFLAGSGTEPWLTDK IRQGVREHKPVLVEILNYKK DGTPFRNAVLVAPIYDDDDE LLYFLGSQVEVDDDQPNMGM ARRERAAEMLKTLSPRQLEV TTLVASGLRNKEVAARLGLS EKTVKMHRGLVMEKLNLKTS ADLVRIAVEAGI

Data sets:
Data typeCount
13C chemical shifts437
15N chemical shifts167
1H chemical shifts655

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1EL222(14-222) protein1
2FMN2

Entities:

Entity 1, EL222(14-222) protein 212 residues - Formula weight is not available

EL222 = Erythrobactor litoralis HTCC2594 protein, 222aa total. GB incorrectly annotated to function and start Met aa. first three residues (GEF) are vector-derived; remainder of sequence are residues 14-222 of protein

1   GLYGLUPHEGLYALAASPASPTHRARGVAL
2   GLUVALGLNPROPROALAGLNTRPVALLEU
3   ASPLEUILEGLUALASERPROILEALASER
4   VALVALSERASPPROARGLEUALAASPASN
5   PROLEUILEALAILEASNGLNALAPHETHR
6   ASPLEUTHRGLYTYRSERGLUGLUGLUCYS
7   VALGLYARGASNCYSARGPHELEUALAGLY
8   SERGLYTHRGLUPROTRPLEUTHRASPLYS
9   ILEARGGLNGLYVALARGGLUHISLYSPRO
10   VALLEUVALGLUILELEUASNTYRLYSLYS
11   ASPGLYTHRPROPHEARGASNALAVALLEU
12   VALALAPROILETYRASPASPASPASPGLU
13   LEULEUTYRPHELEUGLYSERGLNVALGLU
14   VALASPASPASPGLNPROASNMETGLYMET
15   ALAARGARGGLUARGALAALAGLUMETLEU
16   LYSTHRLEUSERPROARGGLNLEUGLUVAL
17   THRTHRLEUVALALASERGLYLEUARGASN
18   LYSGLUVALALAALAARGLEUGLYLEUSER
19   GLULYSTHRVALLYSMETHISARGGLYLEU
20   VALMETGLULYSLEUASNLEULYSTHRSER
21   ALAASPLEUVALARGILEALAVALGLUALA
22   GLYILE

Entity 2, FMN - C17 H21 N4 O9 P - 456.344 Da.

1   FMN

Related Database Links:

PDB 3P7N
GB ABC63043
REF WP_041685679

Download HSQC peak lists in one of the following formats:
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