BMRB Entry 17762

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PDB ID: 2lfj
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR17762
MolProbity Validation Chart

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Title:
Solution structure of the monomeric derivative of BS-RNase
Deposition date:
2011-07-06
Original release date:
2012-01-23
Authors:
Spadaccini, Roberta; Picone, Delia
Citation:

Citation: Spadaccini, Roberta; Ercole, Carmine; Gentile, Maria; Sanfelice, Domenico; Boelens, Rolf; Wechselberger, Rainer; Batta, Gyula; Bernini, Andrea; Niccolai, Neri; Picone, Delia. "NMR Studies on Structure and Dynamics of the Monomeric Derivative of BS-RNase: New Insights for 3D Domain Swapping."  PLoS ONE 7, .-. (2012).
PubMed: 22253705

Assembly members:

Assembly members:
mBS, polymer, 124 residues, 13633.684 Da.

Natural source:

Natural source:   Common Name: cow   Taxonomy ID: 9913   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Bos taurus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET22b(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
mBS: KESAAAKFERQHMDSGNSPS SSSNYCNLMMCCRKMTQGKC KPVNTFVHESLADVKAVCSQ KKVTCKDGQTNCYQSKSTMR ITDCRETGSSKYPNCAYKTT QVEKHIIVACGGKPSVPVHF DASV

Data sets:
Data typeCount
13C chemical shifts350
15N chemical shifts132
1H chemical shifts634

Additional metadata:

  • Assembly
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Assembly:

Entity Assembly IDEntity NameEntity ID
1mBS1

Entities:

Entity 1, mBS 124 residues - 13633.684 Da.

1   LYSGLUSERALAALAALALYSPHEGLUARG
2   GLNHISMETASPSERGLYASNSERPROSER
3   SERSERSERASNTYRCYSASNLEUMETMET
4   CYSCYSARGLYSMETTHRGLNGLYLYSCYS
5   LYSPROVALASNTHRPHEVALHISGLUSER
6   LEUALAASPVALLYSALAVALCYSSERGLN
7   LYSLYSVALTHRCYSLYSASPGLYGLNTHR
8   ASNCYSTYRGLNSERLYSSERTHRMETARG
9   ILETHRASPCYSARGGLUTHRGLYSERSER
10   LYSTYRPROASNCYSALATYRLYSTHRTHR
11   GLNVALGLULYSHISILEILEVALALACYS
12   GLYGLYLYSPROSERVALPROVALHISPHE
13   ASPALASERVAL

Related Database Links:

PDB 11BA 11BG 1BSR 1N1X 1N3Z 1QWQ 1R3M 1R5C 1R5D 1TQ9 1Y92 2LFJ 3BCM 3BCO 3DJO 3DJP 3DJQ 3DJV 3DJX
EMBL CAA04155 CAA26832 CAA35716
GB AAB27820 AAB36140 ELR52955
REF NP_861526 XP_005211518 XP_005900636 XP_010837733
SP P00669
TPE CDG32087
TPG DAA25468
AlphaFold P00669

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