BMRB Entry 17922

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17922

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Title:
Backbone 1H, 13C, 15N assignment of HIV-1 accessory protein Nef
Deposition date:
2011-09-06
Original release date:
2012-09-20
Authors:
Jung, Jinwon; Byeon, In-Ja; Ahn, Jinwoo; Gronenborn, Angela
Citation:

Citation: Jung, Jinwon; Byeon, In-Ja; Ahn, Jinwoo; Gronenborn, Angela. "Structure, dynamics, and Hck interaction of full-length HIV-1 Nef."  Proteins 79, 1609-1622 (2011).
PubMed: 21365684

Assembly members:

Assembly members:
Nef, polymer, 217 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: HIV-1   Taxonomy ID: 11676   Superkingdom: Viruses   Kingdom: not available   Genus/species: Lentivirus HIV-1

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET21

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Nef: MEGKWSKRSVSGWPAVRERM RRAEPAAEGVGAVSRDLEKH GAITSSNTAATNAACAWLEA QEEEEVGFPVRPQVPLRPMT YKAAVDLSHFLKEKGGLEGL IYSQKRQDILDLWVYHTQGY FPDWQNYTPGPGIRYPLTFG WCFKLVPVEPEKVEEANEGE NNCLLHPMSQHGMDDPEKEV LVWKFDSKLAFHHMARELHP EYYKDCAAALEHHHHHH

Data sets:
Data typeCount
13C chemical shifts509
15N chemical shifts150
1H chemical shifts159

Additional metadata:

  • Assembly
  • Samples and Experiments
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  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Nef1

Entities:

Entity 1, Nef 217 residues - Formula weight is not available

1   METGLUGLYLYSTRPSERLYSARGSERVAL
2   SERGLYTRPPROALAVALARGGLUARGMET
3   ARGARGALAGLUPROALAALAGLUGLYVAL
4   GLYALAVALSERARGASPLEUGLULYSHIS
5   GLYALAILETHRSERSERASNTHRALAALA
6   THRASNALAALACYSALATRPLEUGLUALA
7   GLNGLUGLUGLUGLUVALGLYPHEPROVAL
8   ARGPROGLNVALPROLEUARGPROMETTHR
9   TYRLYSALAALAVALASPLEUSERHISPHE
10   LEULYSGLULYSGLYGLYLEUGLUGLYLEU
11   ILETYRSERGLNLYSARGGLNASPILELEU
12   ASPLEUTRPVALTYRHISTHRGLNGLYTYR
13   PHEPROASPTRPGLNASNTYRTHRPROGLY
14   PROGLYILEARGTYRPROLEUTHRPHEGLY
15   TRPCYSPHELYSLEUVALPROVALGLUPRO
16   GLULYSVALGLUGLUALAASNGLUGLYGLU
17   ASNASNCYSLEULEUHISPROMETSERGLN
18   HISGLYMETASPASPPROGLULYSGLUVAL
19   LEUVALTRPLYSPHEASPSERLYSLEUALA
20   PHEHISHISMETALAARGGLULEUHISPRO
21   GLUTYRTYRLYSASPCYSALAALAALALEU
22   GLUHISHISHISHISHISHIS

Related Database Links:

GB AAA03700

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks