BMRB Entry 17946

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17946

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Title:
Aliphatic 1H, 13C, and 15N chemical shift assignments of dihydrofolate reductase from the psychropiezophile Moritella profunda in complex with NADP+ and folate
Deposition date:
2011-09-19
Original release date:
2011-09-23
Authors:
Loveridge, E.; Williams, Christopher; Whittaker, Sara; Gunther, Ulrich; Crump, Matthew; Allemann, Rudolf
Citation:

Citation: Loveridge, E. Joel; Matthews, Stella; Williams, Christopher; Whittaker, Sara B-M; Gunther, Ulrich; Evans, Rhiannon; Dawson, William; Crump, Matthew; Allemann, Rudolf. "Aliphatic (1)H, (13)C and (15)N chemical shift assignments of dihydrofolate reductase from the psychropiezophile Moritella profunda in complex with NADP(+) and folate."  Biomol. NMR Assignments 7, 61-64 (2013).
PubMed: 22415546

Assembly members:

Assembly members:
Dihydrofolate_reductase_from_Moritella_profunda, polymer, 162 residues, 18291.8 Da.
NAP, non-polymer, 743.405 Da.
FOL, non-polymer, 441.397 Da.

Natural source:

Natural source:   Common Name: Moritella profunda   Taxonomy ID: 111291   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Moritella profunda

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pJGetit

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Dihydrofolate_reductase_from_Moritella_profunda: MIVSMIAALANNRVIGLDNK MPWHLPAELQLFKRATLGKP IVMGRNTFESIGRPLPGRLN IVLSRQTDYQPEGVTVVATL EDAVVAAGDVEELMIIGGAT IYNQCLAAADRLYLTHIELT TEGDTWFPDYEQYNWQEIEH ESYAADDKNPHNYRFSLLER VK

Data sets:
Data typeCount
13C chemical shifts665
15N chemical shifts169
1H chemical shifts1062

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Dihydrofolate reductase from Moritella profunda1
2NADP+2
3Folate3

Entities:

Entity 1, Dihydrofolate reductase from Moritella profunda 162 residues - 18291.8 Da.

1   METILEVALSERMETILEALAALALEUALA
2   ASNASNARGVALILEGLYLEUASPASNLYS
3   METPROTRPHISLEUPROALAGLULEUGLN
4   LEUPHELYSARGALATHRLEUGLYLYSPRO
5   ILEVALMETGLYARGASNTHRPHEGLUSER
6   ILEGLYARGPROLEUPROGLYARGLEUASN
7   ILEVALLEUSERARGGLNTHRASPTYRGLN
8   PROGLUGLYVALTHRVALVALALATHRLEU
9   GLUASPALAVALVALALAALAGLYASPVAL
10   GLUGLULEUMETILEILEGLYGLYALATHR
11   ILETYRASNGLNCYSLEUALAALAALAASP
12   ARGLEUTYRLEUTHRHISILEGLULEUTHR
13   THRGLUGLYASPTHRTRPPHEPROASPTYR
14   GLUGLNTYRASNTRPGLNGLUILEGLUHIS
15   GLUSERTYRALAALAASPASPLYSASNPRO
16   HISASNTYRARGPHESERLEULEUGLUARG
17   VALLYS

Entity 2, NADP+ - C21 H28 N7 O17 P3 - 743.405 Da.

1   NAP

Entity 3, Folate - C19 H19 N7 O6 - 441.397 Da.

1   FOL

Related Database Links:

PDB 2ZZA 3IA4 3IA5
DBJ BAI50910 BAO02847
EMBL CAD32276
GB EDM69127
REF WP_043993544

Download HSQC peak lists in one of the following formats:
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