BMRB Entry 18013

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PDB ID: 2lkv
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18013
MolProbity Validation Chart

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Title:
Staphylococcal Nuclease PHS variant
Deposition date:
2011-10-21
Original release date:
2012-06-05
Authors:
Matzapetakis, Manolis; Pais, Tiago; Lamosa, Pedro; Turner, David; Santos, Helena
Citation:

Citation: Pais, Tiago; Lamosa, Pedro; Matzapetakis, Manolis; Turner, David; Santos, Helena. "Mannosylglycerate stabilizes staphylococcal nuclease with restriction of slow -sheet motions."  Protein Sci. 21, 1126-1137 (2012).
PubMed: 22619184

Assembly members:

Assembly members:
SNase_PHS, polymer, 149 residues, 16742.2689 Da.

Natural source:

Natural source:   Common Name: Staphylococcus aureus   Taxonomy ID: 1280   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Staphylococcus aureus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pEt

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SNase_PHS: ATSTKKLHKEPATLIKAIDG DTVKLMYKGQPMTFRLLLVD TPETKHPKKGVEKYGPEASA FTKKMVENAKKIEVEFDKGQ RTDKYGRGLAYIYADGKMVN EALVRQGLAKVAYVYKGNNT HEQLLRKAEAQAKKEKLNIW SEDNADSGQ

Data sets:
Data typeCount
13C chemical shifts642
15N chemical shifts159
1H chemical shifts1046

Additional metadata:

  • Assembly
  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1SNase PHS1

Entities:

Entity 1, SNase PHS 149 residues - 16742.2689 Da.

1   ALATHRSERTHRLYSLYSLEUHISLYSGLU
2   PROALATHRLEUILELYSALAILEASPGLY
3   ASPTHRVALLYSLEUMETTYRLYSGLYGLN
4   PROMETTHRPHEARGLEULEULEUVALASP
5   THRPROGLUTHRLYSHISPROLYSLYSGLY
6   VALGLULYSTYRGLYPROGLUALASERALA
7   PHETHRLYSLYSMETVALGLUASNALALYS
8   LYSILEGLUVALGLUPHEASPLYSGLYGLN
9   ARGTHRASPLYSTYRGLYARGGLYLEUALA
10   TYRILETYRALAASPGLYLYSMETVALASN
11   GLUALALEUVALARGGLNGLYLEUALALYS
12   VALALATYRVALTYRLYSGLYASNASNTHR
13   HISGLUGLNLEULEUARGLYSALAGLUALA
14   GLNALALYSLYSGLULYSLEUASNILETRP
15   SERGLUASPASNALAASPSERGLYGLN

Related Database Links:

BMRB 16585 1704 17718 1874 1875 1876 1877 1878 18788 4010 4052 4053
PDB 1A2T 1A2U 1A3T 1A3U 1A3V 1AEX 1ENA 1ENC 1EY0 1EY4 1EY5 1EY6 1EY7 1EY8 1EY9 1EYA 1EYC 1EYD 1EZ6 1EZ8 1JOK 1JOO 1JOQ 1JOR 1KAA 1KAB 1NUC 1RKN 1SNC 1SNM 1SNO 1SNP 1SNQ 1STB 1STG 1STH 1STN 1STY 1SYC 1SYD 1SYE 1SYF 1SYG 1U9R 2ENB 2EXZ 2EY1 2EY2 2EY5 2EY6 2EYF 2EYH 2EYJ 2EYL 2EYM 2EYO 2EYP 2F0D 2F0E 2F0F 2F0G 2F0H 2F0I 2F0J 2F3V 2F3W 2KHS 2KQ3 2LKV 2M00 2NUC 2OXP 2PW5 2PW7 2PYK 2PZT 2PZU 2PZW 2RKS 2SNM 2SOB 3D6C 3DMU 3NUC 4WOR 5NUC
DBJ BAB41979 BAB56977 BAB94634 BAF67032 BAF77694
EMBL CAA24594 CAG39855 CAG42530 CAI80436 CAQ49298
GB AAC14660 AAW36415 ABD22328 ABD29945 ABE02272
PRF 1109959A 710414A
REF WP_000141556 WP_000141557 WP_001548082 WP_001566557 WP_001574556
SP P00644 Q5HHM4 Q6GB41 Q6GIK1 Q7A6P2
AlphaFold P00644 Q5HHM4 Q6GB41 Q6GIK1 Q7A6P2

Download HSQC peak lists in one of the following formats:
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