BMRB Entry 18039

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PDB ID: 4a5v
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18039
MolProbity Validation Chart

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Title:
Solution structure ensemble of the two N-terminal apple domains (residues 58-231) of Toxoplasma gondii microneme protein 4
Deposition date:
2011-11-01
Original release date:
2012-03-09
Authors:
Marchant, J.; Cowper, B.; Liu, Y.; Lai, L.; Pinzan, C.; Marq, J.; Friedrich, N.; Sawmynaden, K.; Chai, W.; Childs, R.; Saouros, S.; Simpson, P.; Barreira, M.; Feizi, T.; Soldati-favre, D.; Matthews, Stephen
Citation:

Citation: Marchant, Jan; Cowper, Ben; Liu, Yan; Lai, Livia; Pinzan, Camila; Marq, Jean Baptiste; Friedrich, Nikolas; Sawmynaden, Kovilen; Liew, Lloyd; Chai, Wengang; Childs, Robert; Saouros, Savvas; Simpson, Peter; Roque Barreira, Maria Cristina; Feizi, Ten; Soldati-Favre, Dominique; Matthews, Stephen. "Galactose recognition by the apicomplexan parasite Toxoplasma gondii."  J. Biol. Chem. 287, 16720-16733 (2012).
PubMed: 22399295

Assembly members:

Assembly members:
MICRONEMAL_PROTEIN_4, polymer, 161 residues, 17154.4701 Da.

Natural source:

Natural source:   Common Name: Toxoplasma gondii   Taxonomy ID: 5811   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Toxoplasma gondii

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET32 Xa/LIC

Entity Sequences (FASTA):

Entity Sequences (FASTA):
MICRONEMAL_PROTEIN_4: SSEPAKLDLSCVHSDNKGSR APTIGEPVPDVSLEQCAAQC KAVDGCTHFTYNDDSKMCHV KEGKPDLYDLTGGKTASRSC DRSCFEQHVSYEGAPDVMTA MVTSQSADCQAACAADPSCE IFTYNEHDQKCTFKGRGFSA FKERGVLGVTSGPKQFCDEG G

Data sets:
Data typeCount
1H chemical shifts970
13C chemical shifts641
15N chemical shifts161

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Related Database Links:

UNP MIC4_TOXGO
BMRB 15694
PDB 4A5V
AlphaFold Q9XZH7

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SPARKY: Backbone or all simulated peaks