BMRB Entry 18360

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18360

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Title:
The chemical shifts and T1, T2, and 1H-15N NOE data for apo-IscU(E111A)
Deposition date:
2012-03-28
Original release date:
2012-09-14
Authors:
Kim, Jin Hae; Tonelli, Marco; Markley, John
Citation:

Citation: Kim, Jin Hae; Tonelli, Marco; Kim, Taewook; Markley, John. "Three-Dimensional Structure and Determinants of Stability of the Iron-Sulfur Cluster Scaffold Protein IscU from Escherichia coli."  Biochemistry 51, 5557-5563 (2012).
PubMed: 22734684

Assembly members:

Assembly members:
IscU(E111A), polymer, 128 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pTrc99a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
IscU(E111A): MAYSEKVIDHYENPRNVGSF DNNDENVGSGMVGAPACGDV MKLQIKVNDEGIIEDARFKT YGCGSAIASSSLVTEWVKGK SLDEAQAIKNTDIAEELELP PVKIHCSILAADAIKAAIAD YKSKREAK

Data sets:
Data typeCount
13C chemical shifts243
15N chemical shifts80
1H chemical shifts80
T1 relaxation values76
T2 relaxation values75
heteronuclear NOE values76

Additional metadata:

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  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1IscU(E111A)1

Entities:

Entity 1, IscU(E111A) 128 residues - Formula weight is not available

1   METALATYRSERGLULYSVALILEASPHIS
2   TYRGLUASNPROARGASNVALGLYSERPHE
3   ASPASNASNASPGLUASNVALGLYSERGLY
4   METVALGLYALAPROALACYSGLYASPVAL
5   METLYSLEUGLNILELYSVALASNASPGLU
6   GLYILEILEGLUASPALAARGPHELYSTHR
7   TYRGLYCYSGLYSERALAILEALASERSER
8   SERLEUVALTHRGLUTRPVALLYSGLYLYS
9   SERLEUASPGLUALAGLNALAILELYSASN
10   THRASPILEALAGLUGLULEUGLULEUPRO
11   PROVALLYSILEHISCYSSERILELEUALA
12   ALAASPALAILELYSALAALAILEALAASP
13   TYRLYSSERLYSARGGLUALALYS

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