BMRB Entry 18574

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18574

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Title:
1H,13C,15N resonance assignment of wild-type Lipase A from Bacillus subtilis
Deposition date:
2012-07-05
Original release date:
2012-08-29
Authors:
Augustyniak, Wojciech; Wienk, Hans; Boelens, Rolf; Reetz, Manfred
Citation:

Citation: Augustyniak, Wojciech; Brzezinska, Agnieszka; Pijning, Tjaard; Wienk, Hans; Boelens, Rolf; Dijkstra, Bauke; Reetz, Manfred. "Biophysical characterization of mutants of Bacillus subtilis lipase evolved for thermostability: factors contributing to increased activity retention."  Protein Sci. 21, 487-497 (2012).
PubMed: 22267088

Assembly members:

Assembly members:
WT_LipA, polymer, 181 residues, 19362 Da.

Natural source:

Natural source:   Common Name: Firmicutes   Taxonomy ID: 1423   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Bacillus subtilis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-22b(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
WT_LipA: AEHNPVVMVHGIGGASFNFA GIKSYLVSQGWSRDKLYAVD FWDKTGTNYNNGPVLSRFVQ KVLDETGAKKVDIVAHSMGG ANTLYYIKNLDGGNKVANVV TLGGANRLTTGKALPGTDPN QKILYTSIYSSADMIVMNYL SRLDGARNVQIHGVGHIGLL YSSQVNSLIKEGLNGGGQNT N

Data sets:
Data typeCount
13C chemical shifts753
15N chemical shifts195
1H chemical shifts1254

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1WT LipA1

Entities:

Entity 1, WT LipA 181 residues - 19362 Da.

Full-length wild-type Lipase A

1   ALAGLUHISASNPROVALVALMETVALHIS
2   GLYILEGLYGLYALASERPHEASNPHEALA
3   GLYILELYSSERTYRLEUVALSERGLNGLY
4   TRPSERARGASPLYSLEUTYRALAVALASP
5   PHETRPASPLYSTHRGLYTHRASNTYRASN
6   ASNGLYPROVALLEUSERARGPHEVALGLN
7   LYSVALLEUASPGLUTHRGLYALALYSLYS
8   VALASPILEVALALAHISSERMETGLYGLY
9   ALAASNTHRLEUTYRTYRILELYSASNLEU
10   ASPGLYGLYASNLYSVALALAASNVALVAL
11   THRLEUGLYGLYALAASNARGLEUTHRTHR
12   GLYLYSALALEUPROGLYTHRASPPROASN
13   GLNLYSILELEUTYRTHRSERILETYRSER
14   SERALAASPMETILEVALMETASNTYRLEU
15   SERARGLEUASPGLYALAARGASNVALGLN
16   ILEHISGLYVALGLYHISILEGLYLEULEU
17   TYRSERSERGLNVALASNSERLEUILELYS
18   GLUGLYLEUASNGLYGLYGLYGLNASNTHR
19   ASN

Related Database Links:

BMRB 18575
PDB 1I6W 1ISP 1R4Z 1R50 1T2N 1T4M 2QXT 2QXU 3D2A 5CRI 5CT4 5CT5 5CT6 5CT8 5CT9 5CTA 5CUR
DBJ BAA22231 BAI83725 BAM49200 BAM56470 BAP19127
EMBL CAB12064 CCU56724 CEI55388 CEJ75813 CJR84790
GB AAA22574 ABB54395 ABQ08591 ABQ42598 ABQ50891
REF NP_388152 WP_003234767 WP_003246250 WP_014475698 WP_014478727
SP P37957
AlphaFold P37957

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks