BMRB Entry 18575

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18575

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Title:
1H, 13C and 15N resonance assignments of Bacillus subtilis Lipase A mutant evolved towards thermostability
Deposition date:
2012-07-05
Original release date:
2012-08-29
Authors:
Augustyniak, Wojciech; Wienk, Hans; Boelens, Rolf; Reetz, Manfred
Citation:

Citation: Augustyniak, Wojciech; Brzezinska, Agnieszka; Pijning, Tjaard; Wienk, Hans; Boelens, Rolf; Dijkstra, Bauke; Reetz, Manfred. "Biophysical characterization of mutants of Bacillus subtilis lipase evolved for thermostability: factors contributing to increased activity retention."  Protein Sci. 21, 487-497 (2012).
PubMed: 22267088

Assembly members:

Assembly members:
variant_XI_LipA, polymer, 181 residues, 19192 Da.

Natural source:

Natural source:   Common Name: Firmicutes   Taxonomy ID: 1423   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Bacillus subtilis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-22b(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
variant_XI_LipA: AEHNPVVMVHGIGGASFNFA GIKSYLVSQGWSGDKLYAVD FWDKTGTNYNNGPVLSRFVQ KVLDETGAKKVDIVAHSMGG ANTLYYIKNLDGGNKVANVV TLGGANRLTTGDALPGTDPN QKILYTSIYSSADDIVMNCL SRLDGARNVQIHGVGHMGLL YSSQVNSLIKEGLNGGGQNT N

Data sets:
Data typeCount
13C chemical shifts741
15N chemical shifts195
1H chemical shifts1228

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1variant XI LipA1

Entities:

Entity 1, variant XI LipA 181 residues - 19192 Da.

Full length Lipase A variant

1   ALAGLUHISASNPROVALVALMETVALHIS
2   GLYILEGLYGLYALASERPHEASNPHEALA
3   GLYILELYSSERTYRLEUVALSERGLNGLY
4   TRPSERGLYASPLYSLEUTYRALAVALASP
5   PHETRPASPLYSTHRGLYTHRASNTYRASN
6   ASNGLYPROVALLEUSERARGPHEVALGLN
7   LYSVALLEUASPGLUTHRGLYALALYSLYS
8   VALASPILEVALALAHISSERMETGLYGLY
9   ALAASNTHRLEUTYRTYRILELYSASNLEU
10   ASPGLYGLYASNLYSVALALAASNVALVAL
11   THRLEUGLYGLYALAASNARGLEUTHRTHR
12   GLYASPALALEUPROGLYTHRASPPROASN
13   GLNLYSILELEUTYRTHRSERILETYRSER
14   SERALAASPASPILEVALMETASNCYSLEU
15   SERARGLEUASPGLYALAARGASNVALGLN
16   ILEHISGLYVALGLYHISMETGLYLEULEU
17   TYRSERSERGLNVALASNSERLEUILELYS
18   GLUGLYLEUASNGLYGLYGLYGLNASNTHR
19   ASN

Related Database Links:

BMRB 18574
PDB 1I6W 1ISP 1R4Z 1R50 2QXT 2QXU 3QZU 5CRI 5CT4 5CT5 5CT6
DBJ BAA22231 BAI83725 BAM49200 BAM56470 BAP19127
EMBL CAB12064 CEI55388 CEJ75813 CJR84790 COO28547
GB ABQ08591 ABQ42598 ABQ50891 AFN11641 AFQ56205
REF NP_388152 WP_003246250 WP_014478727 WP_019712347 WP_038428323
SP P37957
AlphaFold P37957

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks