BMRB Entry 18606

Click here to enlarge.

PDB ID: 2lw5
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18606
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
Solution structure of anti-CRISPR protein Acr30-35 from Pseudomonas aeruginosa Phage JBD30
Deposition date:
2012-07-22
Original release date:
2013-02-14
Authors:
Maxwell, Karen; Bondy-Denomy, Joe; Davidson, Alan
Citation:

Citation: Bondy-Denomy, Joe; Pawluk, April; Maxwell, Karen; Davidson, Alan. "Bacteriophage genes that inactivate the CRISPR/Cas bacterial immune system."  Nature 493, 429-432 (2013).
PubMed: 23242138

Assembly members:

Assembly members:
entity, polymer, 79 residues, 8825.006 Da.

Natural source:

Natural source:   Common Name: g-proteobacteria   Taxonomy ID: 1223260   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Pseudomonas Pseudomonas Bacteriophage JBD30

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15b-TVL

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: GMKFIKYLSTAHLNYMNIAV YENGSKIKARVENVVNGKSV GARDFDSTEQLESWFYGLPG SGLGRIENAMNEISRRENP

Data sets:
Data typeCount
13C chemical shifts239
15N chemical shifts75
1H chemical shifts509

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1anti-CRISPR protein Acr30-351

Entities:

Entity 1, anti-CRISPR protein Acr30-35 79 residues - 8825.006 Da.

1   GLYMETLYSPHEILELYSTYRLEUSERTHR
2   ALAHISLEUASNTYRMETASNILEALAVAL
3   TYRGLUASNGLYSERLYSILELYSALAARG
4   VALGLUASNVALVALASNGLYLYSSERVAL
5   GLYALAARGASPPHEASPSERTHRGLUGLN
6   LEUGLUSERTRPPHETYRGLYLEUPROGLY
7   SERGLYLEUGLYARGILEGLUASNALAMET
8   ASNGLUILESERARGARGGLUASNPRO

Related Database Links:

PDB 2LW5
DBJ BAR65419
EMBL CDM55208 CRN29922 CRN44629 CRN56640 CRN59456
GB AFE86461 AFQ21949 ERX38869 EZO98398
REF WP_016068276 WP_033975723 YP_006560529 YP_007392342

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks