BMRB Entry 18759

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR18759

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Title:
Backbone 15N, 1H, and CA Chemical Shift Assignments for C186P/A220P IkappaBalpha (67-287)
Deposition date:
2012-10-02
Original release date:
2013-02-07
Authors:
Cervantes, Carla; Handley, Lindsey; Komives, Elizabeth
Citation:

Citation: Cervantes, Carla; Handley, Lindsey; Sue, Shih-Che; Dyson, H. Jane; Komives, Elizabeth. "Long-Range Effects and Functional Consequences of Stabilizing Mutations in the Ankyrin Repeat Domain of IB."  J. Mol. Biol. 425, 902-913 (2013).
PubMed: 23274114

Assembly members:

Assembly members:
CPAP_IkappaBalpha, polymer, 221 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET11a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CPAP_IkappaBalpha: KQQLTEDGDSFLHLAIIHEE KALTMEVIRQVKGDLAFLNF QNNLQQTPLHLAVITNQPEI AEALLGAGCDPELRDFRGNT PLHLACEQGCLASVGVLTQS CTTPHLHSILKATNYNGHTP LHLASIHGYLGIVELLVSLG ADVNAQEPCNGRTPLHLAVD LQNPDLVSLLLKCGADVNRV TYQGYSPYQLTWGRPSTRIQ QQLGQLTLENLQMLPESEDE E

Data sets:
Data typeCount
13C chemical shifts113
15N chemical shifts141
1H chemical shifts141

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1CPAP IkappaBalpha1

Entities:

Entity 1, CPAP IkappaBalpha 221 residues - Formula weight is not available

This is the native human IkappaBalpha (67-287), except with consensus mutations at C186 and A220.

1   LYSGLNGLNLEUTHRGLUASPGLYASPSER
2   PHELEUHISLEUALAILEILEHISGLUGLU
3   LYSALALEUTHRMETGLUVALILEARGGLN
4   VALLYSGLYASPLEUALAPHELEUASNPHE
5   GLNASNASNLEUGLNGLNTHRPROLEUHIS
6   LEUALAVALILETHRASNGLNPROGLUILE
7   ALAGLUALALEULEUGLYALAGLYCYSASP
8   PROGLULEUARGASPPHEARGGLYASNTHR
9   PROLEUHISLEUALACYSGLUGLNGLYCYS
10   LEUALASERVALGLYVALLEUTHRGLNSER
11   CYSTHRTHRPROHISLEUHISSERILELEU
12   LYSALATHRASNTYRASNGLYHISTHRPRO
13   LEUHISLEUALASERILEHISGLYTYRLEU
14   GLYILEVALGLULEULEUVALSERLEUGLY
15   ALAASPVALASNALAGLNGLUPROCYSASN
16   GLYARGTHRPROLEUHISLEUALAVALASP
17   LEUGLNASNPROASPLEUVALSERLEULEU
18   LEULYSCYSGLYALAASPVALASNARGVAL
19   THRTYRGLNGLYTYRSERPROTYRGLNLEU
20   THRTRPGLYARGPROSERTHRARGILEGLN
21   GLNGLNLEUGLYGLNLEUTHRLEUGLUASN
22   LEUGLNMETLEUPROGLUSERGLUASPGLU
23   GLU

Related Database Links:

BMRB 18756 18760
PDB 1IKN 1NFI
DBJ BAE89638 BAG36213 BAI46793
EMBL CAB65556
GB AAA16489 AAH02601 AAH04983 AAK51149 AAP35754
REF NP_001244679 NP_001271861 NP_065390 XP_002753867 XP_002824720
SP P25963
AlphaFold P25963

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks