BMRB Entry 18837

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18837

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Title:
15N, 13C and 1H Resonance Assignments and Secondary Structure Determination of a Variable Heavy Chain Antibody
Deposition date:
2012-11-13
Original release date:
2013-02-28
Authors:
Prosser, Christine; Waters, Lorna; Muskett, Frederick; Veverka, Vaclav; Addis, Philip; Griffin, Laura; Baker, Terry; Lawson, Alastair; Wernery, Ulrich; Kinne, Jorg; Henry, Alistair; Taylor, Richard; Carr, Mark
Citation:

Citation: Prosser, Christine; Waters, Lorna; Muskett, Frederick; Veverka, Vaclav; Addis, Philip; Griffin, Laura; Baker, Alastair; Lawson, Alastair; Wernery, Ulrich; Kinne, Jorg; Henry, Alistair; Taylor, Richard; Carr, Mark. "(15)N, (13)C and (1)H resonance assignments and secondary structure determination of a variable heavy domain of a heavy chain antibody"  Biomol. NMR Assignments ., .-..
PubMed: 23359223

Assembly members:

Assembly members:
VHH_18, polymer, 132 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Camelidae   Taxonomy ID: 9835   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET21

Entity Sequences (FASTA):

Entity Sequences (FASTA):
VHH_18: MHHHHHHENLYFQGEVQLVE SGGGSVQAGGSLRLSCAASG LRISECTTGWYRQAPGKERE LVSKFSNLGTTWYTGSVKGR FTISQDSAKNTVYLQMNSLS PGDTAMYYCNTDLCPWYYEN TWGQGTQVTVSS

Data sets:
Data typeCount
13C chemical shifts422
15N chemical shifts124
1H chemical shifts731

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1VHH 181

Entities:

Entity 1, VHH 18 132 residues - Formula weight is not available

Residues -13 to 0 are the histidine tag and TEV cleavage site. Residues 1-118 are the native sequence

1   METHISHISHISHISHISHISGLUASNLEU
2   TYRPHEGLNGLYGLUVALGLNLEUVALGLU
3   SERGLYGLYGLYSERVALGLNALAGLYGLY
4   SERLEUARGLEUSERCYSALAALASERGLY
5   LEUARGILESERGLUCYSTHRTHRGLYTRP
6   TYRARGGLNALAPROGLYLYSGLUARGGLU
7   LEUVALSERLYSPHESERASNLEUGLYTHR
8   THRTRPTYRTHRGLYSERVALLYSGLYARG
9   PHETHRILESERGLNASPSERALALYSASN
10   THRVALTYRLEUGLNMETASNSERLEUSER
11   PROGLYASPTHRALAMETTYRTYRCYSASN
12   THRASPLEUCYSPROTRPTYRTYRGLUASN
13   THRTRPGLYGLNGLYTHRGLNVALTHRVAL
14   SERSER

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