BMRB Entry 18994

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18994

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Title:
1H, 13C and 15N chemical shift assignments for the N-terminal domain of the KCNH channel from Zebrafish
Deposition date:
2013-01-31
Original release date:
2014-02-03
Authors:
Kim, Young Mee; NG, Hui Qi; Kang, Congbao
Citation:

Citation: Li, Qingxin; Ng, Hui Qi; Kang, Congbao. "(1)H, (13)C and (15)N chemical shift assignments for the cyclic-nucleotide binding homology domain of a KCNH channel."  Biomol. NMR Assignments ., .-. (2014).
PubMed: 24414223

Assembly members:

Assembly members:
zNTD, polymer, 147 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Zebrafish   Taxonomy ID: 7955   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Danio rerio

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET29 b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
zNTD: MPVMRGLLAPQNTFLDTIAT RFDGTHSNFVLGNAQVQSLY PIVYCSDGFCELTGYARAEL MQKSCACHFLYGPETSDRLM AQIQGALDERREFKTELVFY KKGGTQFWCLLDIVPIKNEK GEVVLFLVSHKDITDNKKDL EHHHHHH

Data sets:
Data typeCount
13C chemical shifts538
15N chemical shifts138
1H chemical shifts926

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1zNTD1

Entities:

Entity 1, zNTD 147 residues - Formula weight is not available

Residues 140-147 represent a non-native affinity tag

1   METPROVALMETARGGLYLEULEUALAPRO
2   GLNASNTHRPHELEUASPTHRILEALATHR
3   ARGPHEASPGLYTHRHISSERASNPHEVAL
4   LEUGLYASNALAGLNVALGLNSERLEUTYR
5   PROILEVALTYRCYSSERASPGLYPHECYS
6   GLULEUTHRGLYTYRALAARGALAGLULEU
7   METGLNLYSSERCYSALACYSHISPHELEU
8   TYRGLYPROGLUTHRSERASPARGLEUMET
9   ALAGLNILEGLNGLYALALEUASPGLUARG
10   ARGGLUPHELYSTHRGLULEUVALPHETYR
11   LYSLYSGLYGLYTHRGLNPHETRPCYSLEU
12   LEUASPILEVALPROILELYSASNGLULYS
13   GLYGLUVALVALLEUPHELEUVALSERHIS
14   LYSASPILETHRASPASNLYSLYSASPLEU
15   GLUHISHISHISHISHISHIS

Related Database Links:

REF XP_001919436 XP_007260506 XP_008326080

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