BMRB Entry 19029

Name: 1H, 13C and 15N resonance assignments of K2A1: N-terminal repeat of the K2A domain of Plasmodium falciparum knob - associated histidine rich protein (KAHRP).
Published: 2015-06-05

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19029

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C and 15N resonance assignments of K2A1: N-terminal repeat of the K2A domain of Plasmodium falciparum knob - associated histidine rich protein (KAHRP).

Deposition date:

2013-02-12

Original release date:

2015-06-05

Authors:

Ganguly, Akshay Kumar; Bhavesh, Neel Sarovar

Citation:

Citation: Ganguly, Akshay Kumar; Ranjan, Priyatosh; Kumar, Ashutosh; Bhavesh, Neel Sarovar. "Dynamic association of PfEMP1 and KAHRP in knobs mediates cytoadherence during Plasmodium invasion" Sci Rep. 5, 8617-8617 (2015).
PubMed: 25726759

Assembly members:

Assembly members:
K2A1, polymer, 60 residues, 7028.7 Da.

Natural source:

Natural source: Common Name: Malaria parasite P. falciparum Taxonomy ID: 5833 Superkingdom: Eukaryota Kingdom: not available Genus/species: Plasmodium falciparum

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
K2A1: MGKHHSSKKHEGNDGEGEKK KKSKKHKDHDGEKKKSKKHK DNEDAESVKSKKLEHHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	219
15N chemical shifts	54
1H chemical shifts	216

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	K2A1	1

Entities:

Entity 1, K2A1 60 residues - 7028.7 Da.

The core polypeptide represents the first of two tandem repeats present in the K2A region (5' repeat region) of the Plasmodium falciparum knob associated histidine rich protein. Residues H55 - H60 represent a non - native hexahistidine affinity tag. Residues M1, G2, L53 and E54 are derived from the vector pET28b in which the polypeptide is cloned.

1	MET	GLY	LYS	HIS	HIS	SER	SER	LYS	LYS	HIS
2	GLU	GLY	ASN	ASP	GLY	GLU	GLY	GLU	LYS	LYS
3	LYS	LYS	SER	LYS	LYS	HIS	LYS	ASP	HIS	ASP
4	GLY	GLU	LYS	LYS	LYS	SER	LYS	LYS	HIS	LYS
5	ASP	ASN	GLU	ASP	ALA	GLU	SER	VAL	LYS	SER
6	LYS	LYS	LEU	GLU	HIS	HIS	HIS	HIS	HIS	HIS

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D H(CCCO)NH	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_1	isotropic	sample_conditions_1
2D NCO	sample_1	isotropic	sample_conditions_1
2D CACO	sample_1	isotropic	sample_conditions_1
3D C(CCCO)NH	sample_1	isotropic	sample_conditions_1

EMBL	CAA68268 CAA68270
GB	AAA29629 AAA29630 AAA29632 AAC71810 AAD23574
PRF	1407234A 1413318A 2206359A
REF	XP_001349534
SP	P05229 P06719 P09346 P13817
AlphaFold	P05229 P06719 P09346 P13817

BMRB Entry 19029

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

Related Database Links:

1	MET	GLY	LYS	HIS	HIS	SER	SER	LYS	LYS	HIS
2	GLU	GLY	ASN	ASP	GLY	GLU	GLY	GLU	LYS	LYS
3	LYS	LYS	SER	LYS	LYS	HIS	LYS	ASP	HIS	ASP
4	GLY	GLU	LYS	LYS	LYS	SER	LYS	LYS	HIS	LYS
5	ASP	ASN	GLU	ASP	ALA	GLU	SER	VAL	LYS	SER
6	LYS	LYS	LEU	GLU	HIS	HIS	HIS	HIS	HIS	HIS

1	MET	GLY	LYS	HIS	HIS	SER	SER	LYS	LYS	HIS
2	GLU	GLY	ASN	ASP	GLY	GLU	GLY	GLU	LYS	LYS
3	LYS	LYS	SER	LYS	LYS	HIS	LYS	ASP	HIS	ASP
4	GLY	GLU	LYS	LYS	LYS	SER	LYS	LYS	HIS	LYS
5	ASP	ASN	GLU	ASP	ALA	GLU	SER	VAL	LYS	SER
6	LYS	LYS	LEU	GLU	HIS	HIS	HIS	HIS	HIS	HIS

1	MET	GLY	LYS	HIS	HIS	SER	SER	LYS	LYS	HIS
2	GLU	GLY	ASN	ASP	GLY	GLU	GLY	GLU	LYS	LYS
3	LYS	LYS	SER	LYS	LYS	HIS	LYS	ASP	HIS	ASP
4	GLY	GLU	LYS	LYS	LYS	SER	LYS	LYS	HIS	LYS
5	ASP	ASN	GLU	ASP	ALA	GLU	SER	VAL	LYS	SER
6	LYS	LYS	LEU	GLU	HIS	HIS	HIS	HIS	HIS	HIS