BMRB Entry 19139

Name: Backbone chemical shift assignments of the talin rod domain, R7 (residues 1357-1653 (delta1454-1586))
Published: 2018-06-05

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19139

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone chemical shift assignments of the talin rod domain, R7 (residues 1357-1653 (delta1454-1586))

Deposition date:

2013-04-03

Original release date:

2018-06-05

Authors:

Goult, Benjamin; Bate, Neil; Gingras, Alexandre; Barsukov, Igor; Critchley, David

Citation:

Citation: Bouchet, Benjamin; Gough, Rosemarie; Ammon, York-Christoph; van de Willige, Dieudonnee; Post, Harm; Jacquemet, Guillaume; Altelaar, Af Maarten; Heck, Albert Jr; Goult, Benjamin; Akhmanova, Anna. "Talin-KANK1 interaction controls the recruitment of cortical microtubule stabilizing complexes to focal adhesions" Elife 5, e18124-e18124 (2016).
PubMed: 27410476

Assembly members:

Assembly members:
R7, polymer, 180 residues, 18971 Da.

Natural source:

Natural source: Common Name: House mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pet151-TOPO

Entity Sequences (FASTA):

Entity Sequences (FASTA):
R7: GIDPFTGSAPGQKECDNALR QLETVRELLENPVQPINDMS YFGCLDSVMENSKVLGEAMT GISQNAKNGNLPEFGDAIAT ASKALCGFTEAAAQAAYLVG VSDPNVDQISPEGRAAMEPI VISAKTMLESAGGLIQTARA LAVNPRDPPRWSVLAGHSRT VSDSIKKLITSMRDKAPGQL

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	504
15N chemical shifts	165
1H chemical shifts	165

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	R7	1

Entities:

Entity 1, R7 180 residues - 18971 Da.

Residues 1349-1356 represent a non-native affinity tag. Residues 1454-1584 inclusive deleted from construct.

1	GLY	ILE	ASP	PRO	PHE	THR	GLY	SER	ALA	PRO
2	GLY	GLN	LYS	GLU	CYS	ASP	ASN	ALA	LEU	ARG
3	GLN	LEU	GLU	THR	VAL	ARG	GLU	LEU	LEU	GLU
4	ASN	PRO	VAL	GLN	PRO	ILE	ASN	ASP	MET	SER
5	TYR	PHE	GLY	CYS	LEU	ASP	SER	VAL	MET	GLU
6	ASN	SER	LYS	VAL	LEU	GLY	GLU	ALA	MET	THR
7	GLY	ILE	SER	GLN	ASN	ALA	LYS	ASN	GLY	ASN
8	LEU	PRO	GLU	PHE	GLY	ASP	ALA	ILE	ALA	THR
9	ALA	SER	LYS	ALA	LEU	CYS	GLY	PHE	THR	GLU
10	ALA	ALA	ALA	GLN	ALA	ALA	TYR	LEU	VAL	GLY
11	VAL	SER	ASP	PRO	ASN	VAL	ASP	GLN	ILE	SER
12	PRO	GLU	GLY	ARG	ALA	ALA	MET	GLU	PRO	ILE
13	VAL	ILE	SER	ALA	LYS	THR	MET	LEU	GLU	SER
14	ALA	GLY	GLY	LEU	ILE	GLN	THR	ALA	ARG	ALA
15	LEU	ALA	VAL	ASN	PRO	ARG	ASP	PRO	PRO	ARG
16	TRP	SER	VAL	LEU	ALA	GLY	HIS	SER	ARG	THR
17	VAL	SER	ASP	SER	ILE	LYS	LYS	LEU	ILE	THR
18	SER	MET	ARG	ASP	LYS	ALA	PRO	GLY	GLN	LEU

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks

BMRB Entry 19139

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

1	GLY	ILE	ASP	PRO	PHE	THR	GLY	SER	ALA	PRO
2	GLY	GLN	LYS	GLU	CYS	ASP	ASN	ALA	LEU	ARG
3	GLN	LEU	GLU	THR	VAL	ARG	GLU	LEU	LEU	GLU
4	ASN	PRO	VAL	GLN	PRO	ILE	ASN	ASP	MET	SER
5	TYR	PHE	GLY	CYS	LEU	ASP	SER	VAL	MET	GLU
6	ASN	SER	LYS	VAL	LEU	GLY	GLU	ALA	MET	THR
7	GLY	ILE	SER	GLN	ASN	ALA	LYS	ASN	GLY	ASN
8	LEU	PRO	GLU	PHE	GLY	ASP	ALA	ILE	ALA	THR
9	ALA	SER	LYS	ALA	LEU	CYS	GLY	PHE	THR	GLU
10	ALA	ALA	ALA	GLN	ALA	ALA	TYR	LEU	VAL	GLY
11	VAL	SER	ASP	PRO	ASN	VAL	ASP	GLN	ILE	SER
12	PRO	GLU	GLY	ARG	ALA	ALA	MET	GLU	PRO	ILE
13	VAL	ILE	SER	ALA	LYS	THR	MET	LEU	GLU	SER
14	ALA	GLY	GLY	LEU	ILE	GLN	THR	ALA	ARG	ALA
15	LEU	ALA	VAL	ASN	PRO	ARG	ASP	PRO	PRO	ARG
16	TRP	SER	VAL	LEU	ALA	GLY	HIS	SER	ARG	THR
17	VAL	SER	ASP	SER	ILE	LYS	LYS	LEU	ILE	THR
18	SER	MET	ARG	ASP	LYS	ALA	PRO	GLY	GLN	LEU

1	GLY	ILE	ASP	PRO	PHE	THR	GLY	SER	ALA	PRO
2	GLY	GLN	LYS	GLU	CYS	ASP	ASN	ALA	LEU	ARG
3	GLN	LEU	GLU	THR	VAL	ARG	GLU	LEU	LEU	GLU
4	ASN	PRO	VAL	GLN	PRO	ILE	ASN	ASP	MET	SER
5	TYR	PHE	GLY	CYS	LEU	ASP	SER	VAL	MET	GLU
6	ASN	SER	LYS	VAL	LEU	GLY	GLU	ALA	MET	THR
7	GLY	ILE	SER	GLN	ASN	ALA	LYS	ASN	GLY	ASN
8	LEU	PRO	GLU	PHE	GLY	ASP	ALA	ILE	ALA	THR
9	ALA	SER	LYS	ALA	LEU	CYS	GLY	PHE	THR	GLU
10	ALA	ALA	ALA	GLN	ALA	ALA	TYR	LEU	VAL	GLY
11	VAL	SER	ASP	PRO	ASN	VAL	ASP	GLN	ILE	SER
12	PRO	GLU	GLY	ARG	ALA	ALA	MET	GLU	PRO	ILE
13	VAL	ILE	SER	ALA	LYS	THR	MET	LEU	GLU	SER
14	ALA	GLY	GLY	LEU	ILE	GLN	THR	ALA	ARG	ALA
15	LEU	ALA	VAL	ASN	PRO	ARG	ASP	PRO	PRO	ARG
16	TRP	SER	VAL	LEU	ALA	GLY	HIS	SER	ARG	THR
17	VAL	SER	ASP	SER	ILE	LYS	LYS	LEU	ILE	THR
18	SER	MET	ARG	ASP	LYS	ALA	PRO	GLY	GLN	LEU

1	GLY	ILE	ASP	PRO	PHE	THR	GLY	SER	ALA	PRO
2	GLY	GLN	LYS	GLU	CYS	ASP	ASN	ALA	LEU	ARG
3	GLN	LEU	GLU	THR	VAL	ARG	GLU	LEU	LEU	GLU
4	ASN	PRO	VAL	GLN	PRO	ILE	ASN	ASP	MET	SER
5	TYR	PHE	GLY	CYS	LEU	ASP	SER	VAL	MET	GLU
6	ASN	SER	LYS	VAL	LEU	GLY	GLU	ALA	MET	THR
7	GLY	ILE	SER	GLN	ASN	ALA	LYS	ASN	GLY	ASN
8	LEU	PRO	GLU	PHE	GLY	ASP	ALA	ILE	ALA	THR
9	ALA	SER	LYS	ALA	LEU	CYS	GLY	PHE	THR	GLU
10	ALA	ALA	ALA	GLN	ALA	ALA	TYR	LEU	VAL	GLY
11	VAL	SER	ASP	PRO	ASN	VAL	ASP	GLN	ILE	SER
12	PRO	GLU	GLY	ARG	ALA	ALA	MET	GLU	PRO	ILE
13	VAL	ILE	SER	ALA	LYS	THR	MET	LEU	GLU	SER
14	ALA	GLY	GLY	LEU	ILE	GLN	THR	ALA	ARG	ALA
15	LEU	ALA	VAL	ASN	PRO	ARG	ASP	PRO	PRO	ARG
16	TRP	SER	VAL	LEU	ALA	GLY	HIS	SER	ARG	THR
17	VAL	SER	ASP	SER	ILE	LYS	LYS	LEU	ILE	THR
18	SER	MET	ARG	ASP	LYS	ALA	PRO	GLY	GLN	LEU