BMRB Entry 19191

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19191

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Title:
4EBP1 contains a pre-populated eIF4E-binding helix
Deposition date:
2013-04-25
Original release date:
2013-05-21
Authors:
Han, Kyou-Hoon; Cho, Ye-Jin; Kim, Do-Hyoung; Lee, Chewook; Lee, Si-Hyung; Cha, Eun-Ji; Lim, Ji-Eun; Sabo, T. Michael; Griesinger, Christian; Lee, Donghan
Citation:

Citation: Cho, Ye-Jin; Kim, Do-Hyoung; Lee, Chewook; Lee, Si-Hyung; Cha, Eun-Ji; Lim, Ji-Eun; Sabo, T. Michael; Griesinger, Christian; Lee, Donghan; Han, Kyou-Hoon. "4EBP1 contains a pre-populated eIF4E-binding helix"  Proc. Natl. Acad. Sci. U.S.A. ., .-..

Assembly members:

Assembly members:
eIF4E, polymer, 70 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX2T

Entity Sequences (FASTA):

Entity Sequences (FASTA):
eIF4E: GTRIIYDRKFLMECRNSPVT KTPPRDLPTIPGVTSPSSDE PPMEASQSHLRNSPEDKRAG GEESQFEMDI

Data sets:
Data typeCount
13C chemical shifts262
15N chemical shifts60
1H chemical shifts360

Additional metadata:

  • Assembly
  • Samples and Experiments
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  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1eIF4E1

Entities:

Entity 1, eIF4E 70 residues - Formula weight is not available

1   GLYTHRARGILEILETYRASPARGLYSPHE
2   LEUMETGLUCYSARGASNSERPROVALTHR
3   LYSTHRPROPROARGASPLEUPROTHRILE
4   PROGLYVALTHRSERPROSERSERASPGLU
5   PROPROMETGLUALASERGLNSERHISLEU
6   ARGASNSERPROGLUASPLYSARGALAGLY
7   GLYGLUGLUSERGLNPHEGLUMETASPILE

Related Database Links:

PDB 5BXV
DBJ BAB18650 BAG34949 BAJ20729
EMBL CAG33050
GB AAA62269 AAH04459 AAH58073 AAP35826 AAP36131
PRF 2021415A
REF NP_004086 XP_002819050 XP_004046943 XP_008070572 XP_008954494
SP Q13541
AlphaFold Q13541

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks