BMRB Entry 19330

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19330

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Title:
Sequence Specific Backbone 1H, 15N and 13C assignments of the MAPK binding domain of DUSP 16
Deposition date:
2013-06-28
Original release date:
2013-08-15
Authors:
Senthil Kumar, Ganesan; Peti, Wolfgang; Page, Rebecca
Citation:

Citation: Kumar, Ganesan Senthil; Zettl, Heiko; Page, Rebecca; Peti, Wolfgang. "Structural Basis for the Regulation of the Mitogen-activated Protein (MAP) Kinase p38 by the Dual Specificity Phosphatase 16 MAP Kinase Binding Domain in Solution."  J. Biol. Chem. 288, 28347-28356 (2013).
PubMed: 23926106

Assembly members:

Assembly members:
DUSP16, polymer, 132 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet30a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
DUSP16: GTQIVTERLVALLESGTEKV LLIDSRPFVEYNTSHILEAI NINCSKLMKRRLQQDKVLIT ELIQHSAKHKVDIDCSQKVV VYDQSSQDVASLSSDCFLTV LLGKLEKSFNSVHLLAGGFA EFSRCFPGLCEG

Data sets:
Data typeCount
13C chemical shifts379
15N chemical shifts123
1H chemical shifts123

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1MAPK1

Entities:

Entity 1, MAPK 132 residues - Formula weight is not available

GH - N-terminal sequencing artifact; LEHHHHHH - C-terminal cloning artefact and HIS6-tag

1   GLYTHRGLNILEVALTHRGLUARGLEUVAL
2   ALALEULEUGLUSERGLYTHRGLULYSVAL
3   LEULEUILEASPSERARGPROPHEVALGLU
4   TYRASNTHRSERHISILELEUGLUALAILE
5   ASNILEASNCYSSERLYSLEUMETLYSARG
6   ARGLEUGLNGLNASPLYSVALLEUILETHR
7   GLULEUILEGLNHISSERALALYSHISLYS
8   VALASPILEASPCYSSERGLNLYSVALVAL
9   VALTYRASPGLNSERSERGLNASPVALALA
10   SERLEUSERSERASPCYSPHELEUTHRVAL
11   LEULEUGLYLYSLEUGLULYSSERPHEASN
12   SERVALHISLEULEUALAGLYGLYPHEALA
13   GLUPHESERARGCYSPHEPROGLYLEUCYS
14   GLUGLY

Related Database Links:

PDB 2VSW 3TG3
DBJ BAB21791 BAB40814 BAB47240 BAB71060 BAC29138
GB AAH31643 AAH42101 AAH57321 AAH59232 AAI09235
REF NP_001041519 NP_001100094 NP_085143 NP_569714 XP_001084619
SP Q9BY84
TPG DAA29345
AlphaFold Q9BY84

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks