BMRB Entry 19367
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PDB ID: 2map
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19367
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Campagne, Sebastien; Marsh, May; Capitani, Guido; Vorholt, Julia; Allain, Frederic H-T. "Structural basis for -10 promoter element melting by environmentally induced sigma factors." Nat. Struct. Mol. Biol. 21, 269-276 (2014).
PubMed: 24531660
Assembly members:
entity_1, polymer, 96 residues, 11024.694 Da.
entity_2, polymer, 7 residues, 11024.694 Da.
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET26BII
Entity Sequences (FASTA):
entity_1: MSEQLTDQVLVERVQKGDQK
AFNLLVVRYQHKVASLVSRY
VPSGDVPDVVQEAFIKAYRA
LDSFRGDSAFYTWLYRIAVN
TAKNYLVAQGRRLELV
entity_2: TGTCAAA
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 317 |
| 15N chemical shifts | 103 |
| 1H chemical shifts | 684 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | region 2 of E. coli sigmaE | 1 |
| 2 | non template strand | 2 |
Entities:
Entity 1, region 2 of E. coli sigmaE 96 residues - 11024.694 Da.
| 1 | MET | SER | GLU | GLN | LEU | THR | ASP | GLN | VAL | LEU | ||||
| 2 | VAL | GLU | ARG | VAL | GLN | LYS | GLY | ASP | GLN | LYS | ||||
| 3 | ALA | PHE | ASN | LEU | LEU | VAL | VAL | ARG | TYR | GLN | ||||
| 4 | HIS | LYS | VAL | ALA | SER | LEU | VAL | SER | ARG | TYR | ||||
| 5 | VAL | PRO | SER | GLY | ASP | VAL | PRO | ASP | VAL | VAL | ||||
| 6 | GLN | GLU | ALA | PHE | ILE | LYS | ALA | TYR | ARG | ALA | ||||
| 7 | LEU | ASP | SER | PHE | ARG | GLY | ASP | SER | ALA | PHE | ||||
| 8 | TYR | THR | TRP | LEU | TYR | ARG | ILE | ALA | VAL | ASN | ||||
| 9 | THR | ALA | LYS | ASN | TYR | LEU | VAL | ALA | GLN | GLY | ||||
| 10 | ARG | ARG | LEU | GLU | LEU | VAL |
Entity 2, non template strand 7 residues - 11024.694 Da.
| 1 | DT | DG | DT | DC | DA | DA | DA |
Related Database Links:
| BMRB | 19366 |
| PDB | 1OR7 2MAO 2MAP 4LUP |
| DBJ | BAA10920 BAB36862 BAE76749 BAG78385 BAI26818 |
| EMBL | CAD02789 CAP77019 CAQ32948 CAQ88049 CAQ99523 |
| GB | AAA57186 AAC45314 AAC75626 AAG57689 AAL21534 |
| PIR | AI0829 |
| REF | NP_311466 NP_417068 NP_457116 NP_461575 NP_708425 |
| SP | D0ZSY9 P0A2F0 P0A2F1 P0AGB6 P0AGB7 |
| AlphaFold | D0ZSY9 P0A2F0 P0A2F1 P0AGB6 P0AGB7 |
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CSV: Backbone
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SPARKY: Backbone
or all simulated peaks