BMRB Entry 19429

Name: A bacteriophage transcription regulator inhibits bacterial transcription initiation by -factor displacement
Published: 2014-02-12

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PDB ID: 2mc6
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR19429
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

A bacteriophage transcription regulator inhibits bacterial transcription initiation by -factor displacement

Deposition date:

2013-08-15

Original release date:

2014-02-12

Authors:

Liu, Bing; Shadrin, Andrey; Sheppard, Carol; Xu, Yingqi; Severinov, Konstantin; Matthews, Steve; Wigneshweraraj, Sivaramesh

Citation:

Citation: Liu, Bing; Shadrin, Andrey; Sheppard, Carol; Mekler, Vladimir; Xu, Yingqi; Severinov, Konstantin; Matthews, Steve; Wigneshweraraj, Sivaramesh. "A bacteriophage transcription regulator inhibits bacterial transcription initiation by -factor displacement." Nucleic Acids Res. 42, 4294-4305 (2014).
PubMed: 24482445

Assembly members:

Assembly members:
entity_1, polymer, 67 residues, 8189.045 Da.
entity_2, polymer, 8 residues, 1236.468 Da.

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pet46

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: NEFTQISGYVNAFGSQRGSV LTVKVENDEGWTLVEEDFDR ADYGSDPEFVAEVSSYLKRN GGIKDLT
entity_2: DLLNLFNQ

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	289
15N chemical shifts	66
1H chemical shifts	464

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity_1	1
2	entity_2	2

Entities:

Entity 1, entity_1 67 residues - 8189.045 Da.

1

ASN

GLU

PHE

THR

GLN

ILE

SER

GLY

TYR

VAL

2

ASN

ALA

PHE

GLY

SER

GLN

ARG

GLY

SER

VAL

3

LEU

THR

VAL

LYS

VAL

GLU

ASN

ASP

GLU

GLY

4

TRP

THR

LEU

VAL

GLU

ASP

PHE

ASP

ARG

5

ALA

ASP

TYR

GLY

SER

ASP

PRO

GLU

PHE

VAL

6

ALA

GLU

VAL

SER

TYR

LEU

LYS

ARG

ASN

7

GLY

ILE

LYS

ASP

LEU

THR

Entity 2, entity_2 8 residues - 1236.468 Da.

1

ASP

LEU

ASN

LEU

PHE

ASN

GLN

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H COSY	sample_2	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_2	isotropic	sample_conditions_1

BMRB	19428
PDB	2MC5 2MC6
GB	AAM74072 AAP58713
REF	NP_858993
AlphaFold	Q5GWS7

BMRB Entry 19429

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

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