BMRB Entry 19444

Name: Backbone 1H, 13C, and 15N Chemical Shift Assignments for murine norovirus CR6 NS1/2 protein
Published: 2014-11-21

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PDB ID: 2mck
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19444
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C, and 15N Chemical Shift Assignments for murine norovirus CR6 NS1/2 protein

Deposition date:

2013-08-20

Original release date:

2014-11-21

Authors:

Borin, Brendan; Krezel, Andrzej

Citation:

Citation: Borin, Brendan; Tang, Wei; Nice, Timothy; McCune, Broc; Virgin, Herbert; Krezel, Andrzej. "Murine norovirus protein NS1/2 aspartate to glutamate mutation sufficient for persistence reorients sidechain of surface exposed tryptophan within a novel structured domain" Proteins 82, 1200-1209 (2014).
PubMed: 24273131

Assembly members:

Assembly members:
entity, polymer, 69 residues, 8028.164 Da.

Natural source:

Natural source: Common Name: norovirus Taxonomy ID: 142786 Superkingdom: virus Kingdom: not available Genus/species: norovirus not available

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-BNK

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: MRGSHHHHHHGSGALAALHA DGPHAGLPVTRSDARVLIFN DWEERKRSEPWLRLDMSDKA IFRRYPHLR

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	109
15N chemical shifts	52
1H chemical shifts	379

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	murine norovirus CR6 NS1/2 protein	1

Entities:

Entity 1, murine norovirus CR6 NS1/2 protein 69 residues - 8028.164 Da.

MRGSHHHHHHGS is a non-native affinity tag. The remainder represents residues 58-114 of the native protein.

1

MET

ARG

GLY

SER

HIS

2

GLY

SER

GLY

ALA

LEU

ALA

LEU

HIS

ALA

3

ASP

GLY

PRO

HIS

ALA

GLY

LEU

PRO

VAL

THR

4

ARG

SER

ASP

ALA

ARG

VAL

LEU

ILE

PHE

ASN

5

ASP

TRP

GLU

ARG

LYS

ARG

SER

GLU

PRO

6

TRP

LEU

ARG

LEU

ASP

MET

SER

ASP

LYS

ALA

7

ILE

PHE

ARG

TYR

PRO

HIS

LEU

ARG

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_2	isotropic	sample_conditions_1
CBCANH	sample_2	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1

PDB	2MCK
GB	ABU55606 AEY83582

BMRB Entry 19444

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

Related Database Links: