BMRB Entry 19507

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19507

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Title:
Backbone assignment and Secondary Structure of Intrinsically Unstructured Culture Filtrate Antigen protein (CFP10) from Mycobacterium Tuberculosis
Deposition date:
2013-09-19
Original release date:
2013-11-11
Authors:
Pandey, Himanshu; Guleria, Anupam; Raikwal, Nisha; Arora, Ashish; Kumar, Dinesh
Citation:

Citation: Guleria, Anupam; Raikwal, Nisha; Shukla, Vaibhav; Pandey, Himanshu; Arora, Ashish; Kumar, Dinesh. "Pseudo 5D HN(C)N Experiment to Facilitate the Assignment of Backbone Resonances in Proteins Exhibiting High Backbone Shift Degeneracy"  .

Assembly members:

Assembly members:
CFP10, polymer, 113 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Mycobacterium tuberculosis   Taxonomy ID: 1773   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Mycobacterium tuberculosis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CFP10: MAEMKTDAATLAQEAGNFER ISGDLKTQIDQVESTAGSLQ GQWRGAAGTAAQAAVVRFQE AANKQKQELDEISTNIRQAG VQYSRADEEQQQALSSQMGF KLAAALEHHHHHH

Data sets:
Data typeCount
13C chemical shifts317
15N chemical shifts109
1H chemical shifts109

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1CFP10 Monomer1

Entities:

Entity 1, CFP10 Monomer 113 residues - Formula weight is not available

1   METALAGLUMETLYSTHRASPALAALATHR
2   LEUALAGLNGLUALAGLYASNPHEGLUARG
3   ILESERGLYASPLEULYSTHRGLNILEASP
4   GLNVALGLUSERTHRALAGLYSERLEUGLN
5   GLYGLNTRPARGGLYALAALAGLYTHRALA
6   ALAGLNALAALAVALVALARGPHEGLNGLU
7   ALAALAASNLYSGLNLYSGLNGLULEUASP
8   GLUILESERTHRASNILEARGGLNALAGLY
9   VALGLNTYRSERARGALAASPGLUGLUGLN
10   GLNGLNALALEUSERSERGLNMETGLYPHE
11   LYSLEUALAALAALALEUGLUHISHISHIS
12   HISHISHIS

Related Database Links:

PDB 1WA8 3FAV
DBJ BAL68012 BAQ08111 GAA43756
EMBL CCC28957 CCC46227 CCE39297 CCG13791 CCK53890
GB AAC83445 AAK48356 AAL14999 ABQ75702 ABR08234
REF NP_218391 NP_857541 WP_003399940 WP_003900771 WP_009937166
SP P0A567 P9WNK4 P9WNK5
AlphaFold P0A567 P9WNK4 P9WNK5

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks