BMRB Entry 19589

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19589

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Title:
NMR assignment of the RhoGAP domain from the Rgd1 protein of Saccharomyces cerevisiae
Deposition date:
2013-10-29
Original release date:
2014-11-10
Authors:
Martinez, Denis; Prouzet-mauleon, Valerie; Hugues, Michel; Claveres, Annie; Doignon, Francois; Odaert, Benoit
Citation:

Citation: Martinez, Denis; Prouzet-Mauleon, Valerie; Hugues, Michel; Doignon, Francois; Odaert, Benoit. "Assignment of 1H, 13C and 15N resonances and secondary structure of the Rgd1-RhoGAP domain"  Biomol. NMR Assign. 12, 129-132 (2018).
PubMed: 29280056

Assembly members:

Assembly members:
Rgd1p-RhoGAP_domain, polymer, 226 residues, 25692 Da.

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET21a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Rgd1p-RhoGAP_domain: MISHIQTNNNMPPGVQKNFK TFGVPLESLIEFEQDMVPAI VRQCIYVIDKFGLDQEGIYR KSANVLDVSKLKEEIDKDPA NISMILPSKPHSDSDIYLVG SLLKTFFASLPDSVLPKALS SEIKVCLQIEDPTTRKNFMH GLIYNLPDAQYWTLRALVFH LKRVLAHEAQNRMNLRALCI IWGPTIAPANPDDANDVNFQ IMAMEVLLEVSDQAFEPELE HHHHHH

Data sets:
Data typeCount
13C chemical shifts853
15N chemical shifts210
1H chemical shifts1431

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1RhoGAP1

Entities:

Entity 1, RhoGAP 226 residues - 25692 Da.

The His-tag involve residues 219 to 226.

1   METILESERHISILEGLNTHRASNASNASN
2   METPROPROGLYVALGLNLYSASNPHELYS
3   THRPHEGLYVALPROLEUGLUSERLEUILE
4   GLUPHEGLUGLNASPMETVALPROALAILE
5   VALARGGLNCYSILETYRVALILEASPLYS
6   PHEGLYLEUASPGLNGLUGLYILETYRARG
7   LYSSERALAASNVALLEUASPVALSERLYS
8   LEULYSGLUGLUILEASPLYSASPPROALA
9   ASNILESERMETILELEUPROSERLYSPRO
10   HISSERASPSERASPILETYRLEUVALGLY
11   SERLEULEULYSTHRPHEPHEALASERLEU
12   PROASPSERVALLEUPROLYSALALEUSER
13   SERGLUILELYSVALCYSLEUGLNILEGLU
14   ASPPROTHRTHRARGLYSASNPHEMETHIS
15   GLYLEUILETYRASNLEUPROASPALAGLN
16   TYRTRPTHRLEUARGALALEUVALPHEHIS
17   LEULYSARGVALLEUALAHISGLUALAGLN
18   ASNARGMETASNLEUARGALALEUCYSILE
19   ILETRPGLYPROTHRILEALAPROALAASN
20   PROASPASPALAASNASPVALASNPHEGLN
21   ILEMETALAMETGLUVALLEULEUGLUVAL
22   SERASPGLNALAPHEGLUPROGLULEUGLU
23   HISHISHISHISHISHIS

Related Database Links:

SP P38339
AlphaFold D6VQQ7

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks