BMRB Entry 19635

Name: Solution structure of TpsB4 N-terminal POTRA domain from Pseudomonas aeruginosa
Published: 2014-12-22

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PDB ID: 2mhj
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19635
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of TpsB4 N-terminal POTRA domain from Pseudomonas aeruginosa

Deposition date:

2013-11-25

Original release date:

2014-12-22

Authors:

Garnett, James; Matthews, Steve

Citation:

Citation: Garnett, James; Matthews, Steve. "Structure-function analysis reveals a dual role of the Pseudomonas aeruginosa Tps4 two-partner secretion system" .

Assembly members:

Assembly members:
TpsB4, polymer, 95 residues, 10764.220 Da.

Natural source:

Natural source: Common Name: g-proteobacteria Taxonomy ID: 287 Superkingdom: Bacteria Kingdom: not available Genus/species: Pseudomonas aeruginosa

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET46 LIC/Ek

Entity Sequences (FASTA):

Entity Sequences (FASTA):
TpsB4: MAHHHHHHVDDDDKMGGPTV LVKRFQLSGNRQFDDRRLLA LLHDLPGQELNLSQLHAAAA RIGDFYQEKGYVLARAFLPA QEIQDGTVRIEVLEG

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	388
15N chemical shifts	83
1H chemical shifts	607

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	TpsB4	1

Entities:

Entity 1, TpsB4 95 residues - 10764.220 Da.

Residues 1-15 represent a non-native affinity tag. Residues 16-95 correspond to residues 42-121 of mature TpsB4.

1

MET

ALA

HIS

VAL

ASP

2

ASP

LYS

MET

GLY

PRO

THR

VAL

3

LEU

VAL

LYS

ARG

PHE

GLN

LEU

SER

GLY

ASN

4

ARG

GLN

PHE

ASP

ARG

LEU

ALA

5

LEU

HIS

ASP

LEU

PRO

GLY

GLN

GLU

LEU

6

ASN

LEU

SER

GLN

LEU

HIS

ALA

7

ARG

ILE

GLY

ASP

PHE

TYR

GLN

GLU

LYS

GLY

8

TYR

VAL

LEU

ALA

ARG

ALA

PHE

LEU

PRO

ALA

9

GLN

GLU

ILE

GLN

ASP

GLY

THR

VAL

ARG

ILE

10

GLU

VAL

LEU

GLU

GLY

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	sample_2	isotropic	sample_conditions_1
2D 1H-13C HSQC aromatic	sample_2	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_2	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	sample_2	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1

DBJ	BAK87931 BAP21481 BAP53040 BAQ42187 BAR69849
EMBL	CAW29677 CCQ83911 CDH73159 CDH79418 CDI95309
GB	AAG07928 ABJ13808 AEO77112 AFM66993 AGI83581
REF	NP_253230 WP_003094678 WP_003112835 WP_003120951 WP_003122077

BMRB Entry 19635

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

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