BMRB Entry 19778

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PDB ID: 2mkk
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19778
MolProbity Validation Chart

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Title:
Structural model of tandem RRM domains of cytoplasmic polyadenylation element binding protein 1 (CPEB1) in complex with RNA
Deposition date:
2014-02-07
Original release date:
2014-07-21
Authors:
Afroz, Tariq; Skrisovska, Lenka; Belloc, Eulalia; Boixet, Jordina; Mendez, Raul; Allain, Frederic
Citation:

Citation: Afroz, Tariq; Skrisovska, Lenka; Belloc, Eulalia; Boixet, Jordina; Mendez, Raul; Allain, Frederic. "A fly trap mechanism provides sequence-specific RNA recognition by CPEB proteins"  Genes Dev. 28, 1498-1514 (2014).
PubMed: 24990967

Assembly members:

Assembly members:
CPEB1RRM12, polymer, 213 residues, 23930.746 Da.
5'-UUUUA-3', polymer, 5 residues, 1508.924 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28A(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CPEB1RRM12: MTWSGQLPPRNYKNPIYSCK VFLGGVPWDITEAGLVNTFR VFGSLSVEWPGKDGKHPRCP PKGYVYLVFELEKSVRSLLQ ACSHDPLSPDGLSEYYFKMS SRRMRCKEVQVIPWVLADSN FVRSPSQRLDPSRTVFVGAL HGMLNAEALAAILNDLFGGV VYAGIDTDKHKYPIGSGRVT FNNQRSYLKAVSAAFVEIKT TKFTKKVQIDPYL
5'-UUUUA-3': UUUUA

Data sets:
Data typeCount

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1CPEB1RRM121
2RNA (5'-R(*UP*UP*UP*UP*A)-3')2

Entities:

Entity 1, CPEB1RRM12 213 residues - 23930.746 Da.

1   METTHRTRPSERGLYGLNLEUPROPROARG
2   ASNTYRLYSASNPROILETYRSERCYSLYS
3   VALPHELEUGLYGLYVALPROTRPASPILE
4   THRGLUALAGLYLEUVALASNTHRPHEARG
5   VALPHEGLYSERLEUSERVALGLUTRPPRO
6   GLYLYSASPGLYLYSHISPROARGCYSPRO
7   PROLYSGLYTYRVALTYRLEUVALPHEGLU
8   LEUGLULYSSERVALARGSERLEULEUGLN
9   ALACYSSERHISASPPROLEUSERPROASP
10   GLYLEUSERGLUTYRTYRPHELYSMETSER
11   SERARGARGMETARGCYSLYSGLUVALGLN
12   VALILEPROTRPVALLEUALAASPSERASN
13   PHEVALARGSERPROSERGLNARGLEUASP
14   PROSERARGTHRVALPHEVALGLYALALEU
15   HISGLYMETLEUASNALAGLUALALEUALA
16   ALAILELEUASNASPLEUPHEGLYGLYVAL
17   VALTYRALAGLYILEASPTHRASPLYSHIS
18   LYSTYRPROILEGLYSERGLYARGVALTHR
19   PHEASNASNGLNARGSERTYRLEULYSALA
20   VALSERALAALAPHEVALGLUILELYSTHR
21   THRLYSPHETHRLYSLYSVALGLNILEASP
22   PROTYRLEU

Entity 2, RNA (5'-R(*UP*UP*UP*UP*A)-3') 5 residues - 1508.924 Da.

1   UUUUA

Related Database Links:

BMRB 19775
PDB 2MKH 2MKK
DBJ BAB14496 BAE87429 BAG63194 BAH11723 BAH13212
EMBL CAH92427
GB AAH35348 AAH50629 AAK01239 AAK01240 ADQ32841
REF NP_001073001 NP_001073002 NP_001073003 NP_001126432 NP_001239455
SP Q5R733 Q9BZB8
TPG DAA17635
AlphaFold Q5R733 Q9BZB8

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