BMRB Entry 19968

Name: 1H, 13C, and 15N resonance assignments for the tandem PHD finger motifs of human CHD4
Published: 2016-10-06

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19968

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C, and 15N resonance assignments for the tandem PHD finger motifs of human CHD4

Deposition date:

2014-05-12

Original release date:

2016-10-06

Authors:

Walport, Louise; Morra, Rosa; Mancini, Erika; Redfield, Christina

Citation:

Citation: Walport, Louise; Morra, Rosa; Mancini, Erika; Redfield, Christina. "(1)H, (13)C, and (15)N resonance assignments for the tandem PHD finger motifs of human CHD4" Biomol. NMR Assign. 9, 239-242 (2015).
PubMed: 25326197

Assembly members:

Assembly members:
dPHD, polymer, 148 residues, Formula weight is not available
ZINC ION, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pTriEx2

Entity Sequences (FASTA):

Entity Sequences (FASTA):
dPHD: METDHQDYCEVCQQGGEIIL CDTCPRAYHMVCLDPDMEKA PEGKWSCPHCEKEGIQWEAK EDNSEGEEILEEVGGDPEEE DDHHMEFCRVCKDGGELLCC DTCPSSYHIHCLNPPLPEIP NGEWLCPRCTCPALKGKKLE HHHHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	474
15N chemical shifts	128
1H chemical shifts	790

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	dPHD	1
2	Zinc1	2
3	Zinc2	2
4	Zinc3	2
5	Zinc4	2

Entities:

Entity 1, dPHD 148 residues - Formula weight is not available

Residues 130-7 represent a non-native affinity tag

1

MET

GLU

THR

ASP

HIS

GLN

ASP

TYR

CYS

GLU

2

VAL

CYS

GLN

GLY

GLU

ILE

LEU

3

CYS

ASP

THR

CYS

PRO

ARG

ALA

TYR

HIS

MET

4

VAL

CYS

LEU

ASP

PRO

ASP

MET

GLU

LYS

ALA

5

PRO

GLU

GLY

LYS

TRP

SER

CYS

PRO

HIS

CYS

6

GLU

LYS

GLU

GLY

ILE

GLN

TRP

GLU

ALA

LYS

7

GLU

ASP

ASN

SER

GLU

GLY

GLU

ILE

LEU

8

GLU

VAL

GLY

ASP

PRO

GLU

9

ASP

HIS

MET

GLU

PHE

CYS

ARG

VAL

10

CYS

LYS

ASP

GLY

GLU

LEU

CYS

11

ASP

THR

CYS

PRO

SER

TYR

HIS

ILE

HIS

12

CYS

LEU

ASN

PRO

LEU

PRO

GLU

ILE

PRO

13

ASN

GLY

GLU

TRP

LEU

CYS

PRO

ARG

CYS

THR

14

CYS

PRO

ALA

LEU

LYS

GLY

LYS

LEU

GLU

15

HIS

Entity 2, Zinc1 - Zn - 65.409 Da.

1

ZN

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_2	isotropic	sample_conditions_1
3D HN(CO)CA	sample_2	isotropic	sample_conditions_1
3D CBCANH	sample_2	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_2	isotropic	sample_conditions_1
3D HNCO	sample_2	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_2	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_2	isotropic	sample_conditions_1
3D H(CCO)NH	sample_2	isotropic	sample_conditions_1
3D (H)C(CO)NH	sample_2	isotropic	sample_conditions_1
3D NH(CA)CO	sample_2	isotropic	sample_conditions_1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks

BMRB Entry 19968

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers: