BMRB Entry 19977

Click here to enlarge.

PDB ID: 2mpf
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19977
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
Solution structure human HCN2 CNBD in the cAMP-unbound state
Deposition date:
2014-05-16
Original release date:
2014-09-02
Authors:
Saponaro, Andrea; Pauleta, Sofia; Cantini, Francesca; Matzapetakis, Manolis; Hammann, Christian; Banci, Lucia; Thiel, Gerard; Santoro, Bina; Moroni, Anna
Citation:

Citation: Saponaro, Andrea; Pauleta, Sofia; Cantini, Francesca; Matzapetakis, Manolis; Hammann, Christian; Banci, Lucia; Thiel, Gerard; Santoro, Bina; Moroni, Anna. "Structural basis for the mutual antagonism of cAMP and TRIP8b in regulating HCN channel function"  .

Assembly members:

Assembly members:
entity, polymer, 158 residues, 15918.672 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-24b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: GPSSPMELNGPLREEIVNFN CRKLVASMPLFANADPNFVT AMLTKLKFEVFQPGDYIIRE GTIGKKMYFIQHGVVSVLTK GNKEMKLSDGSYFGEICLLT RGRRTASVRADTYCRLYSLS VDNFNEVLEEYPMMRRAFET VAIDRLDRIGKKNSILLH

Data sets:
Data typeCount
1H chemical shifts1113
13C chemical shifts624
15N chemical shifts149

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1human HCN2 CNBD1

Entities:

Entity 1, human HCN2 CNBD 158 residues - 15918.672 Da.

Residues 515-520 represent a non-native affinity tag Residues 521-533 are unstructured and were omitted for simplicity

1   GLYPROSERSERPROMETGLULEUASNGLY
2   PROLEUARGGLUGLUILEVALASNPHEASN
3   CYSARGLYSLEUVALALASERMETPROLEU
4   PHEALAASNALAASPPROASNPHEVALTHR
5   ALAMETLEUTHRLYSLEULYSPHEGLUVAL
6   PHEGLNPROGLYASPTYRILEILEARGGLU
7   GLYTHRILEGLYLYSLYSMETTYRPHEILE
8   GLNHISGLYVALVALSERVALLEUTHRLYS
9   GLYASNLYSGLUMETLYSLEUSERASPGLY
10   SERTYRPHEGLYGLUILECYSLEULEUTHR
11   ARGGLYARGARGTHRALASERVALARGALA
12   ASPTHRTYRCYSARGLEUTYRSERLEUSER
13   VALASPASNPHEASNGLUVALLEUGLUGLU
14   TYRPROMETMETARGARGALAPHEGLUTHR
15   VALALAILEASPARGLEUASPARGILEGLY
16   LYSLYSASNSERILELEULEUHIS

Related Database Links:

PDB 1Q3E 2MPF 2Q0A 3BPZ 3ETQ 3FFQ 3U10
DBJ BAD32628 BAI44633 BAI45607
EMBL CAA12406 CAB42602 CAB42630
GB AAC28444 AAC39760 AAC40125 AAF62174 AAI18002
REF NP_001185 NP_032252 NP_446136 XP_002193445 XP_002689207
SP O88703 Q9JKA9 Q9UL51
TPG DAA27526
AlphaFold O88703 Q9JKA9 Q9UL51

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks