BMRB Entry 25019

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR25019

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Title:
Sequential backbone HN, N, Ca and Cb assignments of E.coli dihydrofolate reductase-folate binary complex at pH 7.0 and 308 K
Deposition date:
2014-06-13
Original release date:
2014-07-14
Authors:
Puthenpurackal Narayanan, Sunilkumar; Maeno, Akihiro; Wada, Yuji; Tate, Shin-ichi; Akasaka, Kazuyuki
Citation:

Citation: Puthenpurackal Narayanan, Sunilkumar; Maeno, Akihiro; Wada, Yuji; Tate, Shin-ichi; Akasaka, Kazuyuki. "Sequential backbone resonance assignments of the E. coli dihydrofolate reductase Gly67Val mutant: folate complex"  Biomol. NMR Assign. ., .-. (2015).
PubMed: 26482924

Assembly members:

Assembly members:
DHFR, polymer, 159 residues, Formula weight is not available
entity_FOL, non-polymer, 441.397 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pTP64-1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
DHFR: MISLIAALAVDRVIGMENAM PWNLPADLAWFKRNTLNKPV IMGRHTWESIGRPLPGRKNI ILSSQPGTDDRVTWVKSVDE AIAACGDVPEIMVIGGGRVY EQFLPKAQKLYLTHIDAEVE GDTHFPDYEPDDWESVFSEF HDADAQNSHSYCFEILERR

Data sets:
Data typeCount
13C chemical shifts295
15N chemical shifts140
1H chemical shifts140

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1DHFR1
2folate2

Entities:

Entity 1, DHFR 159 residues - Formula weight is not available

1   METILESERLEUILEALAALALEUALAVAL
2   ASPARGVALILEGLYMETGLUASNALAMET
3   PROTRPASNLEUPROALAASPLEUALATRP
4   PHELYSARGASNTHRLEUASNLYSPROVAL
5   ILEMETGLYARGHISTHRTRPGLUSERILE
6   GLYARGPROLEUPROGLYARGLYSASNILE
7   ILELEUSERSERGLNPROGLYTHRASPASP
8   ARGVALTHRTRPVALLYSSERVALASPGLU
9   ALAILEALAALACYSGLYASPVALPROGLU
10   ILEMETVALILEGLYGLYGLYARGVALTYR
11   GLUGLNPHELEUPROLYSALAGLNLYSLEU
12   TYRLEUTHRHISILEASPALAGLUVALGLU
13   GLYASPTHRHISPHEPROASPTYRGLUPRO
14   ASPASPTRPGLUSERVALPHESERGLUPHE
15   HISASPALAASPALAGLNASNSERHISSER
16   TYRCYSPHEGLUILELEUGLUARGARG

Entity 2, folate - C19 H19 N7 O6 - 441.397 Da.

1   FOL

Related Database Links:

BMRB 11492 4554
PDB 1DDR 1DDS 1DHI 1DHJ 1DRA 1DRB 1DRE 1DRH 1DYH 1DYI 1DYJ 1JOL 1JOM 1RA1 1RA2 1RA3 1RA8 1RA9 1RB2 1RB3 1RC4 1RD7 1RE7 1RF7 1RG7 1RH3 1RX1 1RX2 1RX3 1RX4 1RX5 1RX6 1RX7 1RX8 1RX9 1TDR 2ANO 2ANQ 2DRC 2INQ 3DAU 3DRC 3K74 3KFY 3OCH 3QL0 3QL3 3QYL 3QYO 3R33 4DFR 4EIG 4EIZ 4EJ1 4FHB 4I13 4I1N 4KJJ 4KJK 4KJL 4NX6 4NX7 4P3Q 4P3R 4P66 4P68 4PSS 4PST 4PSY 4PTH 4PTJ 4QLE 4QLF 4QLG 4RGC 5CC9 5CCC 5DFR 6DFR 7DFR
DBJ BAA05974 BAB33474 BAB96616 BAG75573 BAI23410
EMBL CAA28755 CAD01243 CAP74618 CAQ30568 CAQ87642
GB AAA87976 AAC73159 AAG54351 AAN41711 AAN78554
PIR AC0513
REF NP_308078 NP_414590 NP_454699 NP_706004 WP_000378105
SP P0ABQ4 P0ABQ5 P0ABQ6
AlphaFold P0ABQ4 P0ABQ5 P0ABQ6

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks