BMRB Entry 25077

Name: Backbone 1H, 13C, and 15N Chemical Shift Assignments for the PDZ domain and the linker of the Protein Tyrosine phosphatase non-receptor type 4
Published: 2014-09-19

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25077

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C, and 15N Chemical Shift Assignments for the PDZ domain and the linker of the Protein Tyrosine phosphatase non-receptor type 4

Deposition date:

2014-07-04

Original release date:

2014-09-19

Authors:

Maisonneuve, Pierre; Caillet-Saguy, Celia; Babault, Nicolas; Cordier, Florence; wolff, Nicolas

Citation:

Citation: Maisonneuve, Pierre; Caillet-Saguy, Celia; Raynal, Bertrand; Gilquin, Bernard; Chaffotte, Alain; Perez, Javier; Zinn-Justin, Sophie; Delepierre, Muriel; Buc, Henri; Cordier, Florence; Wolff, Nicolas. "Regulation of the Catalytic Activity of the Human Phosphatase PTPN4 by Its PDZ Domain." FEBS J. ., .-. (2014).
PubMed: 25158884

Assembly members:

Assembly members:
Protein-tyrosine_phosphatase_4, polymer, 140 residues, 15604.6 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pDEST15

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Protein-tyrosine_phosphatase_4: MSSPEKPTPNGGIPHDNLVL IRMKPDENGRFGFNVKGGYD QKMPVIVSRVAPGTPADLCV PRLNEGDQVVLINGRDIAEH THDQVVLFIKASCERHSGEL MLLVRPNAVYDVVEEKLENE PDFQYIPEKAPLDSVHQDDH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1
heteronucl_T1_relaxation
- heteronuclear_T1_list_1
heteronucl_T2_relaxation
- heteronuclear_T2_list_1

Data type	Count
13C chemical shifts	367
1H chemical shifts	116
15N chemical shifts	116
T1 relaxation values	107
T2 relaxation values	103

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	PTPN4 PDZ-linker	1

Entities:

Entity 1, PTPN4 PDZ-linker 140 residues - 15604.6 Da.

Residue 1 is a non residue due to TEV cleavage site

1	MET	SER	SER	PRO	GLU	LYS	PRO	THR	PRO	ASN
2	GLY	GLY	ILE	PRO	HIS	ASP	ASN	LEU	VAL	LEU
3	ILE	ARG	MET	LYS	PRO	ASP	GLU	ASN	GLY	ARG
4	PHE	GLY	PHE	ASN	VAL	LYS	GLY	GLY	TYR	ASP
5	GLN	LYS	MET	PRO	VAL	ILE	VAL	SER	ARG	VAL
6	ALA	PRO	GLY	THR	PRO	ALA	ASP	LEU	CYS	VAL
7	PRO	ARG	LEU	ASN	GLU	GLY	ASP	GLN	VAL	VAL
8	LEU	ILE	ASN	GLY	ARG	ASP	ILE	ALA	GLU	HIS
9	THR	HIS	ASP	GLN	VAL	VAL	LEU	PHE	ILE	LYS
10	ALA	SER	CYS	GLU	ARG	HIS	SER	GLY	GLU	LEU
11	MET	LEU	LEU	VAL	ARG	PRO	ASN	ALA	VAL	TYR
12	ASP	VAL	VAL	GLU	GLU	LYS	LEU	GLU	ASN	GLU
13	PRO	ASP	PHE	GLN	TYR	ILE	PRO	GLU	LYS	ALA
14	PRO	LEU	ASP	SER	VAL	HIS	GLN	ASP	ASP	HIS

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_2	isotropic	sample_conditions_2

EMBL	P29074
PDB	2CS5 2VPH 3NFK 3NFL
DBJ	BAG37355 BAH13435
GB	AAA36530 AAH10674 AAY24016 ADZ15528 AIC49534
REF	NP_001093949 NP_001253553 NP_002821 XP_002712421 XP_002749576
SP	P29074
AlphaFold	P29074

BMRB Entry 25077

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

Related Database Links:

1	MET	SER	SER	PRO	GLU	LYS	PRO	THR	PRO	ASN
2	GLY	GLY	ILE	PRO	HIS	ASP	ASN	LEU	VAL	LEU
3	ILE	ARG	MET	LYS	PRO	ASP	GLU	ASN	GLY	ARG
4	PHE	GLY	PHE	ASN	VAL	LYS	GLY	GLY	TYR	ASP
5	GLN	LYS	MET	PRO	VAL	ILE	VAL	SER	ARG	VAL
6	ALA	PRO	GLY	THR	PRO	ALA	ASP	LEU	CYS	VAL
7	PRO	ARG	LEU	ASN	GLU	GLY	ASP	GLN	VAL	VAL
8	LEU	ILE	ASN	GLY	ARG	ASP	ILE	ALA	GLU	HIS
9	THR	HIS	ASP	GLN	VAL	VAL	LEU	PHE	ILE	LYS
10	ALA	SER	CYS	GLU	ARG	HIS	SER	GLY	GLU	LEU
11	MET	LEU	LEU	VAL	ARG	PRO	ASN	ALA	VAL	TYR
12	ASP	VAL	VAL	GLU	GLU	LYS	LEU	GLU	ASN	GLU
13	PRO	ASP	PHE	GLN	TYR	ILE	PRO	GLU	LYS	ALA
14	PRO	LEU	ASP	SER	VAL	HIS	GLN	ASP	ASP	HIS

1	MET	SER	SER	PRO	GLU	LYS	PRO	THR	PRO	ASN
2	GLY	GLY	ILE	PRO	HIS	ASP	ASN	LEU	VAL	LEU
3	ILE	ARG	MET	LYS	PRO	ASP	GLU	ASN	GLY	ARG
4	PHE	GLY	PHE	ASN	VAL	LYS	GLY	GLY	TYR	ASP
5	GLN	LYS	MET	PRO	VAL	ILE	VAL	SER	ARG	VAL
6	ALA	PRO	GLY	THR	PRO	ALA	ASP	LEU	CYS	VAL
7	PRO	ARG	LEU	ASN	GLU	GLY	ASP	GLN	VAL	VAL
8	LEU	ILE	ASN	GLY	ARG	ASP	ILE	ALA	GLU	HIS
9	THR	HIS	ASP	GLN	VAL	VAL	LEU	PHE	ILE	LYS
10	ALA	SER	CYS	GLU	ARG	HIS	SER	GLY	GLU	LEU
11	MET	LEU	LEU	VAL	ARG	PRO	ASN	ALA	VAL	TYR
12	ASP	VAL	VAL	GLU	GLU	LYS	LEU	GLU	ASN	GLU
13	PRO	ASP	PHE	GLN	TYR	ILE	PRO	GLU	LYS	ALA
14	PRO	LEU	ASP	SER	VAL	HIS	GLN	ASP	ASP	HIS

1	MET	SER	SER	PRO	GLU	LYS	PRO	THR	PRO	ASN
2	GLY	GLY	ILE	PRO	HIS	ASP	ASN	LEU	VAL	LEU
3	ILE	ARG	MET	LYS	PRO	ASP	GLU	ASN	GLY	ARG
4	PHE	GLY	PHE	ASN	VAL	LYS	GLY	GLY	TYR	ASP
5	GLN	LYS	MET	PRO	VAL	ILE	VAL	SER	ARG	VAL
6	ALA	PRO	GLY	THR	PRO	ALA	ASP	LEU	CYS	VAL
7	PRO	ARG	LEU	ASN	GLU	GLY	ASP	GLN	VAL	VAL
8	LEU	ILE	ASN	GLY	ARG	ASP	ILE	ALA	GLU	HIS
9	THR	HIS	ASP	GLN	VAL	VAL	LEU	PHE	ILE	LYS
10	ALA	SER	CYS	GLU	ARG	HIS	SER	GLY	GLU	LEU
11	MET	LEU	LEU	VAL	ARG	PRO	ASN	ALA	VAL	TYR
12	ASP	VAL	VAL	GLU	GLU	LYS	LEU	GLU	ASN	GLU
13	PRO	ASP	PHE	GLN	TYR	ILE	PRO	GLU	LYS	ALA
14	PRO	LEU	ASP	SER	VAL	HIS	GLN	ASP	ASP	HIS