BMRB Entry 25473

Assembly:

Entities:

Entity 1, Pcf11 96 residues - Formula weight is not available

Contains N-terminal Gly-His-Met residues following cleavage of the His6-tag by TEV protease

Samples:

sample_1: Pcf11, [U-99% 15N], 450 uM; sodium phosphate 20 mM; sodium chloride 50 mM; D2O, [U-100% 2H], 10%; H2O 90%; DTT 2 mM

sample_2: Pcf11, [U-99% 13C; U-99% 15N], 200 uM; sodium phosphate 20 mM; sodium chloride 150 mM; D2O, [U-100% 2H], 10%; H2O 90%; DTT 2 mM

sample_3: Pcf11, [U-99% 13C; U-99% 15N], 200 uM; sodium phosphate 20 mM; sodium chloride 150 mM; D2O, [U-100% 2H], 100%; DTT 2 mM

sample_4: Pcf11, [U-10% 13C], 100 uM; sodium phosphate 20 mM; sodium chloride 150 mM; D2O, [U-100% 2H], 10%; H2O 90%; DTT 2 mM

sample_5: Pcf11, [U-13C; U-15N]-Leu, 200 uM; sodium phosphate 20 mM; sodium chloride 150 mM; D2O, [U-100% 2H], 100%; DTT 2 mM

sample_conditions_1: ionic strength: 170 mM; pH: 6.5; pressure: 1 atm; temperature: 300 K

sample_conditions_2: ionic strength: 70 mM; pH: 6.5; pressure: 1 atm; temperature: 300 K

Experiments:

Software:

TOPSPIN, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment, data analysis

NMR spectrometers:

• Bruker Avance 800 MHz
• Bruker Avance 700 MHz