BMRB Entry 25488
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PDB ID: 2mzh
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25488
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Prior, Stephen; Fulcher, Yan; Koppisetti, Rama; Jurkevich, A.; Van Doren, Steven. "Charge-Triggered Membrane Insertion of Matrix Metalloproteinase-7, Supporter of Innate Immunity and Tumors" Structure 23, 2099-2110 (2015).
PubMed: 26439767
Assembly members:
proMMP-7(E195A), polymer, 248 residues, 27702.492 Da.
CALCIUM ION, non-polymer, 40.078 Da.
ZINC ION, non-polymer, 65.409 Da.
1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE, non-polymer, 678.940 Da.
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28-a
Entity Sequences (FASTA):
proMMP-7(E195A): LPQEAGGMSELQWEQAQDYL
KRFYLYDSETKNANSLEAKL
KEMQKFFGLPITGMLNSRVI
EIMQKPRCGVPDVAEYSLFP
NSPKWTSKVVTYRIVSYTRD
LPHITVDRLVSKALNMWGKE
IPLHFRKVVWGTADIMIGFA
RGAHGDSYPFDGPGNTLAHA
FAPGTGLGGDAHFDEDERWT
DGSSLGINFLYAATHALGHS
LGMGHSSDPNAVMYPTYGNG
DPQNFKLSQDDIKGIQKLYG
KRSNSRKK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 62 |
| 15N chemical shifts | 223 |
| 1H chemical shifts | 1218 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity_1 | 1 |
| 2 | CALCIUM ION_1 | 2 |
| 3 | ZINC ION_1 | 3 |
| 4 | 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE_1 | 4 |
Entities:
Entity 1, entity_1 248 residues - 27702.492 Da.
| 1 | LEU | PRO | GLN | GLU | ALA | GLY | GLY | MET | SER | GLU | ||||
| 2 | LEU | GLN | TRP | GLU | GLN | ALA | GLN | ASP | TYR | LEU | ||||
| 3 | LYS | ARG | PHE | TYR | LEU | TYR | ASP | SER | GLU | THR | ||||
| 4 | LYS | ASN | ALA | ASN | SER | LEU | GLU | ALA | LYS | LEU | ||||
| 5 | LYS | GLU | MET | GLN | LYS | PHE | PHE | GLY | LEU | PRO | ||||
| 6 | ILE | THR | GLY | MET | LEU | ASN | SER | ARG | VAL | ILE | ||||
| 7 | GLU | ILE | MET | GLN | LYS | PRO | ARG | CYS | GLY | VAL | ||||
| 8 | PRO | ASP | VAL | ALA | GLU | TYR | SER | LEU | PHE | PRO | ||||
| 9 | ASN | SER | PRO | LYS | TRP | THR | SER | LYS | VAL | VAL | ||||
| 10 | THR | TYR | ARG | ILE | VAL | SER | TYR | THR | ARG | ASP | ||||
| 11 | LEU | PRO | HIS | ILE | THR | VAL | ASP | ARG | LEU | VAL | ||||
| 12 | SER | LYS | ALA | LEU | ASN | MET | TRP | GLY | LYS | GLU | ||||
| 13 | ILE | PRO | LEU | HIS | PHE | ARG | LYS | VAL | VAL | TRP | ||||
| 14 | GLY | THR | ALA | ASP | ILE | MET | ILE | GLY | PHE | ALA | ||||
| 15 | ARG | GLY | ALA | HIS | GLY | ASP | SER | TYR | PRO | PHE | ||||
| 16 | ASP | GLY | PRO | GLY | ASN | THR | LEU | ALA | HIS | ALA | ||||
| 17 | PHE | ALA | PRO | GLY | THR | GLY | LEU | GLY | GLY | ASP | ||||
| 18 | ALA | HIS | PHE | ASP | GLU | ASP | GLU | ARG | TRP | THR | ||||
| 19 | ASP | GLY | SER | SER | LEU | GLY | ILE | ASN | PHE | LEU | ||||
| 20 | TYR | ALA | ALA | THR | HIS | ALA | LEU | GLY | HIS | SER | ||||
| 21 | LEU | GLY | MET | GLY | HIS | SER | SER | ASP | PRO | ASN | ||||
| 22 | ALA | VAL | MET | TYR | PRO | THR | TYR | GLY | ASN | GLY | ||||
| 23 | ASP | PRO | GLN | ASN | PHE | LYS | LEU | SER | GLN | ASP | ||||
| 24 | ASP | ILE | LYS | GLY | ILE | GLN | LYS | LEU | TYR | GLY | ||||
| 25 | LYS | ARG | SER | ASN | SER | ARG | LYS | LYS |
Entity 2, CALCIUM ION_1 - Ca - 40.078 Da.
| 1 | CA |
Entity 3, ZINC ION_1 - Zn - 65.409 Da.
| 1 | ZN |
Entity 4, 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE_1 - C36 H73 N O8 P - 678.940 Da.
| 1 | PX4 |
Related Database Links:
| NCBI | NP_002414.1 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks