BMRB Entry 25697

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25697

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Title:
human CFTR NBD1 deltaRI I539T mutant chemical shift assignments
Deposition date:
2015-07-09
Original release date:
2015-08-12
Authors:
Chong, Andrew; Forman-Kay, Julie
Citation:

Citation: Chong, Andrew; Farber, Patrick; Vernon, Robert; Hudson, Rhea; Mittermaier, Anthony; Forman-Kay, Julie. "Deletion of Phenylalanine-508 in the First Nucleotide Binding Domain of the Cystic Fibrosis Transmembrane Conductance Regulator Increases Conformational Exchange and Inhibits Dimerization"  J. Biol. Chem. 290, 22862-22878 (2015).
PubMed: 26149808

Assembly members:

Assembly members:
hCFTR_NBD1_delta_RI_I539T, polymer, 229 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-SUMO hNBD1 deltaRI I539T

Entity Sequences (FASTA):

Entity Sequences (FASTA):
hCFTR_NBD1_delta_RI_I539T: SLTTTEVVMENVTAFWEEGG TPVLKDINFKIERGQLLAVA GSTGAGKTSLLMMIMGELEP SEGKIKHSGRISFCSQFSWI MPGTIKENIIFGVSYDEYRY RSVIKACQLEEDISKFAEKD NTVLGEGGITLSGGQRARIS LARAVYKDADLYLLDSPFGY LDVLTEKEIFESCVCKLMAN KTRILVTSKMEHLKKADKIL ILHEGSSYFYGTFSELQNLQ PDFSSKLMG

Data sets:
Data typeCount
13C chemical shifts581
15N chemical shifts206
1H chemical shifts220

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1hCFTR NBD1 delta RI I539T1

Entities:

Entity 1, hCFTR NBD1 delta RI I539T 229 residues - Formula weight is not available

1   SERLEUTHRTHRTHRGLUVALVALMETGLU
2   ASNVALTHRALAPHETRPGLUGLUGLYGLY
3   THRPROVALLEULYSASPILEASNPHELYS
4   ILEGLUARGGLYGLNLEULEUALAVALALA
5   GLYSERTHRGLYALAGLYLYSTHRSERLEU
6   LEUMETMETILEMETGLYGLULEUGLUPRO
7   SERGLUGLYLYSILELYSHISSERGLYARG
8   ILESERPHECYSSERGLNPHESERTRPILE
9   METPROGLYTHRILELYSGLUASNILEILE
10   PHEGLYVALSERTYRASPGLUTYRARGTYR
11   ARGSERVALILELYSALACYSGLNLEUGLU
12   GLUASPILESERLYSPHEALAGLULYSASP
13   ASNTHRVALLEUGLYGLUGLYGLYILETHR
14   LEUSERGLYGLYGLNARGALAARGILESER
15   LEUALAARGALAVALTYRLYSASPALAASP
16   LEUTYRLEULEUASPSERPROPHEGLYTYR
17   LEUASPVALLEUTHRGLULYSGLUILEPHE
18   GLUSERCYSVALCYSLYSLEUMETALAASN
19   LYSTHRARGILELEUVALTHRSERLYSMET
20   GLUHISLEULYSLYSALAASPLYSILELEU
21   ILELEUHISGLUGLYSERSERTYRPHETYR
22   GLYTHRPHESERGLULEUGLNASNLEUGLN
23   PROASPPHESERSERLYSLEUMETGLY

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