BMRB Entry 25698

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR25698

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
human CFTR NBD1 deltaRI F508del mutant chemical shift assignments
Deposition date:
2015-07-09
Original release date:
2015-08-12
Authors:
Hudson, Rhea; Chong, Andrew; Forman-Kay, Julie
Citation:

Citation: Chong, Andrew; Farber, Patrick; Vernon, Robert; Hudson, Rhea; Mittermaier, Anthony; Forman-Kay, Julie. "Deletion of Phenylalanine-508 in the First Nucleotide Binding Domain of the Cystic Fibrosis Transmembrane Conductance Regulator Increases Conformational Exchange and Inhibits Dimerization"  J. Biol. Chem. 290, 22862-22878 (2015).
PubMed: 26149808

Assembly members:

Assembly members:
hCFTR_NBD1_delta_RI_F508del, polymer, 228 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-SUMO hNBD1 deltaRI F508del

Entity Sequences (FASTA):

Entity Sequences (FASTA):
hCFTR_NBD1_delta_RI_F508del: SLTTTEVVMENVTAFWEEGG TPVLKDINFKIERGQLLAVA GSTGAGKTSLLMMIMGELEP SEGKIKHSGRISFCSQFSWI MPGTIKENIIGVSYDEYRYR SVIKACQLEEDISKFAEKDN IVLGEGGITLSGGQRARISL ARAVYKDADLYLLDSPFGYL DVLTEKEIFESCVCKLMANK TRILVTSKMEHLKKADKILI LHEGSSYFYGTFSELQNLQP DFSSKLMG

Data sets:
Data typeCount
13C chemical shifts375
15N chemical shifts186
1H chemical shifts185

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1hCFTR NBD1 delta RI F508del1

Entities:

Entity 1, hCFTR NBD1 delta RI F508del 228 residues - Formula weight is not available

1   SERLEUTHRTHRTHRGLUVALVALMETGLU
2   ASNVALTHRALAPHETRPGLUGLUGLYGLY
3   THRPROVALLEULYSASPILEASNPHELYS
4   ILEGLUARGGLYGLNLEULEUALAVALALA
5   GLYSERTHRGLYALAGLYLYSTHRSERLEU
6   LEUMETMETILEMETGLYGLULEUGLUPRO
7   SERGLUGLYLYSILELYSHISSERGLYARG
8   ILESERPHECYSSERGLNPHESERTRPILE
9   METPROGLYTHRILELYSGLUASNILEILE
10   GLYVALSERTYRASPGLUTYRARGTYRARG
11   SERVALILELYSALACYSGLNLEUGLUGLU
12   ASPILESERLYSPHEALAGLULYSASPASN
13   ILEVALLEUGLYGLUGLYGLYILETHRLEU
14   SERGLYGLYGLNARGALAARGILESERLEU
15   ALAARGALAVALTYRLYSASPALAASPLEU
16   TYRLEULEUASPSERPROPHEGLYTYRLEU
17   ASPVALLEUTHRGLULYSGLUILEPHEGLU
18   SERCYSVALCYSLYSLEUMETALAASNLYS
19   THRARGILELEUVALTHRSERLYSMETGLU
20   HISLEULYSLYSALAASPLYSILELEUILE
21   LEUHISGLUGLYSERSERTYRPHETYRGLY
22   THRPHESERGLULEUGLNASNLEUGLNPRO
23   ASPPHESERSERLYSLEUMETGLY

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks