BMRB Entry 25730
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25730
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Citation: Amin, Nader; Chiarparin, Elisabetta; Coyle, Joe; Nietlispach, Daniel; Williams, Glyn. "1H, 15N and 13C backbone assignments of GDP-bound human H-Ras mutant G12V" Biomol. NMR Assign. 10, 121-123.
PubMed: 26373425
Assembly members:
H-Ras_G12V, polymer, 169 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET23b
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 482 |
| 15N chemical shifts | 160 |
| 1H chemical shifts | 160 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | H-Ras g12V | 1 |
Entities:
Entity 1, H-Ras g12V 169 residues - Formula weight is not available
| 1 | GLY | SER | HIS | MET | THR | GLU | TYR | LYS | LEU | VAL | ||||
| 2 | VAL | VAL | GLY | ALA | VAL | GLY | VAL | GLY | LYS | SER | ||||
| 3 | ALA | LEU | THR | ILE | GLN | LEU | ILE | GLN | ASN | HIS | ||||
| 4 | PHE | VAL | ASP | GLU | TYR | ASP | PRO | THR | ILE | GLU | ||||
| 5 | ASP | SER | TYR | ARG | LYS | GLN | VAL | VAL | ILE | ASP | ||||
| 6 | GLY | GLU | THR | CYS | LEU | LEU | ASP | ILE | LEU | ASP | ||||
| 7 | THR | ALA | GLY | GLN | GLU | GLU | TYR | SER | ALA | MET | ||||
| 8 | ARG | ASP | GLN | TYR | MET | ARG | THR | GLY | GLU | GLY | ||||
| 9 | PHE | LEU | CYS | VAL | PHE | ALA | ILE | ASN | ASN | THR | ||||
| 10 | LYS | SER | PHE | GLU | ASP | ILE | HIS | GLN | TYR | ARG | ||||
| 11 | GLU | GLN | ILE | LYS | ARG | VAL | LYS | ASP | SER | ASP | ||||
| 12 | ASP | VAL | PRO | MET | VAL | LEU | VAL | GLY | ASN | LYS | ||||
| 13 | CYS | ASP | LEU | ALA | ALA | ARG | THR | VAL | GLU | SER | ||||
| 14 | ARG | GLN | ALA | GLN | ASP | LEU | ALA | ARG | SER | TYR | ||||
| 15 | GLY | ILE | PRO | TYR | ILE | GLU | THR | SER | ALA | LYS | ||||
| 16 | THR | ARG | GLN | GLY | VAL | GLU | ASP | ALA | PHE | TYR | ||||
| 17 | THR | LEU | VAL | ARG | GLU | ILE | ARG | GLN | HIS |
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CSV: Backbone
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SPARKY: Backbone
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