BMRB Entry 26048
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26048
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Citation: Alexandropoulos, Ioannis; Argyriou, Aikaterini; Maroyusis, Konstantinos; Topouzis, Stavros; Papapetropoulos, Andreas; Spyroulias, Georgios. "(1)H, (13)C, (15)N backbone and side-chain resonance assignment of Nostoc sp. C139A variant of the heme-nitric oxide/oxygen binding (H-NOX) domain" Biomol. NMR Assign. 10, 395-400 (2016).
PubMed: 27614467
Assembly members:
nostoc_sp_H-NOX_(C139A), polymer, 183 residues, 20380 Da.
PROTOPORPHYRIN IX CONTAINING FE, non-polymer, 616.487 Da.
Natural source: Common Name: cyanobacteria Taxonomy ID: 1172 Superkingdom: Bacteria Kingdom: not available Genus/species: Anabaena variabilis
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET.22b(+)
Entity Sequences (FASTA):
nostoc_sp_H-NOX_(C139A): MYGLVNKAIQDMISKHHGED
TWEAIKQKAGLEDIDFFVGM
EAYSDDVTYHLVGAASEVLG
KPAEELLIAFGEYWVTYTSE
EGYGELLASAGDSLPEFMEN
LDNLHARVGLSFPQLRPPAF
ECQHTSSKSMELHYQSTRAG
LAPMVLGLLHGLGKRFQTKV
EVTQTAFRETGEDHDIFSIK
YED
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 603 |
| 15N chemical shifts | 149 |
| 1H chemical shifts | 917 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | H-NOX Monomer | 1 |
| 2 | HEM | 2 |
Entities:
Entity 1, H-NOX Monomer 183 residues - 20380 Da.
| 1 | MET | TYR | GLY | LEU | VAL | ASN | LYS | ALA | ILE | GLN | ||||
| 2 | ASP | MET | ILE | SER | LYS | HIS | HIS | GLY | GLU | ASP | ||||
| 3 | THR | TRP | GLU | ALA | ILE | LYS | GLN | LYS | ALA | GLY | ||||
| 4 | LEU | GLU | ASP | ILE | ASP | PHE | PHE | VAL | GLY | MET | ||||
| 5 | GLU | ALA | TYR | SER | ASP | ASP | VAL | THR | TYR | HIS | ||||
| 6 | LEU | VAL | GLY | ALA | ALA | SER | GLU | VAL | LEU | GLY | ||||
| 7 | LYS | PRO | ALA | GLU | GLU | LEU | LEU | ILE | ALA | PHE | ||||
| 8 | GLY | GLU | TYR | TRP | VAL | THR | TYR | THR | SER | GLU | ||||
| 9 | GLU | GLY | TYR | GLY | GLU | LEU | LEU | ALA | SER | ALA | ||||
| 10 | GLY | ASP | SER | LEU | PRO | GLU | PHE | MET | GLU | ASN | ||||
| 11 | LEU | ASP | ASN | LEU | HIS | ALA | ARG | VAL | GLY | LEU | ||||
| 12 | SER | PHE | PRO | GLN | LEU | ARG | PRO | PRO | ALA | PHE | ||||
| 13 | GLU | CYS | GLN | HIS | THR | SER | SER | LYS | SER | MET | ||||
| 14 | GLU | LEU | HIS | TYR | GLN | SER | THR | ARG | ALA | GLY | ||||
| 15 | LEU | ALA | PRO | MET | VAL | LEU | GLY | LEU | LEU | HIS | ||||
| 16 | GLY | LEU | GLY | LYS | ARG | PHE | GLN | THR | LYS | VAL | ||||
| 17 | GLU | VAL | THR | GLN | THR | ALA | PHE | ARG | GLU | THR | ||||
| 18 | GLY | GLU | ASP | HIS | ASP | ILE | PHE | SER | ILE | LYS | ||||
| 19 | TYR | GLU | ASP |
Entity 2, HEM - C34 H32 Fe N4 O4 - 616.487 Da.
| 1 | HEM |
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