BMRB Entry 26647

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26647

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Title:
NFkB p65 DNA-binding domain
Deposition date:
2015-09-02
Original release date:
2016-09-02
Authors:
Mukherjee, Sulakshana; Quintas, Pedro; Borin, Brendan
Citation:

Citation: Mukherjee, Sulakshana; Borin, Brendan; Quintas, Pedro; Dyson, Jane. "NMR characterization of a 72 kDa transcription factor using differential isotopic labeling"  Protein Sci. 25, 597-604 (2016).
PubMed: 26647230

Assembly members:

Assembly members:
p65DBD, polymer, 173 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET

Entity Sequences (FASTA):

Entity Sequences (FASTA):
p65DBD: PYVEIIEQPKQRGMRFRYKC EGRSAGSIPGERSTDTTKTH PTIKINGYTGPGTVRISLVT KDPPHRPHPHELVGKDCRDG YYEADLCPDRSIHSFQNLGI QCVKKRDLEQAISQRIQTNN NPFHVPIEEQRGDYDLNAVR LCFQVTVRDPAGRPLLLTPV LSHPIFDNRAPNT

Data sets:
Data typeCount
13C chemical shifts296
15N chemical shifts151
1H chemical shifts499

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1p65 DBD1

Entities:

Entity 1, p65 DBD 173 residues - Formula weight is not available

1   PROTYRVALGLUILEILEGLUGLNPROLYS
2   GLNARGGLYMETARGPHEARGTYRLYSCYS
3   GLUGLYARGSERALAGLYSERILEPROGLY
4   GLUARGSERTHRASPTHRTHRLYSTHRHIS
5   PROTHRILELYSILEASNGLYTYRTHRGLY
6   PROGLYTHRVALARGILESERLEUVALTHR
7   LYSASPPROPROHISARGPROHISPROHIS
8   GLULEUVALGLYLYSASPCYSARGASPGLY
9   TYRTYRGLUALAASPLEUCYSPROASPARG
10   SERILEHISSERPHEGLNASNLEUGLYILE
11   GLNCYSVALLYSLYSARGASPLEUGLUGLN
12   ALAILESERGLNARGILEGLNTHRASNASN
13   ASNPROPHEHISVALPROILEGLUGLUGLN
14   ARGGLYASPTYRASPLEUASNALAVALARG
15   LEUCYSPHEGLNVALTHRVALARGASPPRO
16   ALAGLYARGPROLEULEULEUTHRPROVAL
17   LEUSERHISPROILEPHEASPASNARGALA
18   PROASNTHR

Related Database Links:

PDB 1NFI 1VKX

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks