BMRB Entry 26658

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26658

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for SczA from Streptococcus agalactiae
Deposition date:
2015-09-15
Original release date:
2017-03-03
Authors:
Edmonds, Katherine; Giedroc, David
Citation:

Citation: Martin, Julia; Edmonds, Katherine; Bruce, Kevin; Campanello, Gregory; Eijkelkamp, Bart; Brazel, Erin; McDevitt, Christopher; Winkler, Malcolm; Giedroc, David. "The zinc efflux activator SczA protects Streptococcus pneumoniae serotype 2 D39 from intracellular zinc toxicity"  Mol. Microbiol. 104, 636-651 (2017).
PubMed: 28249108

Assembly members:

Assembly members:
SczA_transcriptional_regulator, polymer, 174 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: firmicutes   Taxonomy ID: 1311   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Streptococcus agalactiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET3a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SczA_transcriptional_regulator: MVKDRQIQKTKVAIYNAFIS LLQENDYSKITVQDVIGLAN VGRSTFYSHYESKEVLLKEL CEDLFHHLFKQGRDVTFEEY LVHILKHFEQNQDSIATLLL SDDPYFLLRFRSELEHDVYP RLREEYITKVDIPEDFLKQF LLSSFIETLKWWLHQRQKMT VEDLLKYYLTMVER

Data sets:
Data typeCount
13C chemical shifts461
15N chemical shifts157
1H chemical shifts157

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SczA, chain 11
2SczA, chain 21

Entities:

Entity 1, SczA, chain 1 174 residues - Formula weight is not available

1   METVALLYSASPARGGLNILEGLNLYSTHR
2   LYSVALALAILETYRASNALAPHEILESER
3   LEULEUGLNGLUASNASPTYRSERLYSILE
4   THRVALGLNASPVALILEGLYLEUALAASN
5   VALGLYARGSERTHRPHETYRSERHISTYR
6   GLUSERLYSGLUVALLEULEULYSGLULEU
7   CYSGLUASPLEUPHEHISHISLEUPHELYS
8   GLNGLYARGASPVALTHRPHEGLUGLUTYR
9   LEUVALHISILELEULYSHISPHEGLUGLN
10   ASNGLNASPSERILEALATHRLEULEULEU
11   SERASPASPPROTYRPHELEULEUARGPHE
12   ARGSERGLULEUGLUHISASPVALTYRPRO
13   ARGLEUARGGLUGLUTYRILETHRLYSVAL
14   ASPILEPROGLUASPPHELEULYSGLNPHE
15   LEULEUSERSERPHEILEGLUTHRLEULYS
16   TRPTRPLEUHISGLNARGGLNLYSMETTHR
17   VALGLUASPLEULEULYSTYRTYRLEUTHR
18   METVALGLUARG

Related Database Links:

PDB 3KKC
NCBI WP_000237802

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks